IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v12y2021i1d10.1038_s41467-021-21686-3.html
   My bibliography  Save this article

Global profiling of distinct cysteine redox forms reveals wide-ranging redox regulation in C. elegans

Author

Listed:
  • Jin Meng

    (Joslin Diabetes Center
    Harvard Medical School
    Harvard Stem Cell Institute)

  • Ling Fu

    (Beijing Institute of Lifeomics
    Qingdao University)

  • Keke Liu

    (Beijing Institute of Lifeomics)

  • Caiping Tian

    (Beijing Institute of Lifeomics
    Tsinghua University)

  • Ziyun Wu

    (Joslin Diabetes Center
    Harvard Medical School
    Harvard Stem Cell Institute)

  • Youngeun Jung

    (The Scripps Research Institute)

  • Renan B. Ferreira

    (The Scripps Research Institute)

  • Kate S. Carroll

    (The Scripps Research Institute)

  • T. Keith Blackwell

    (Joslin Diabetes Center
    Harvard Medical School
    Harvard Stem Cell Institute)

  • Jing Yang

    (Beijing Institute of Lifeomics
    Qingdao University)

Abstract

Post-translational changes in the redox state of cysteine residues can rapidly and reversibly alter protein functions, thereby modulating biological processes. The nematode C. elegans is an ideal model organism for studying cysteine-mediated redox signaling at a network level. Here we present a comprehensive, quantitative, and site-specific profile of the intrinsic reactivity of the cysteinome in wild-type C. elegans. We also describe a global characterization of the C. elegans redoxome in which we measured changes in three major cysteine redox forms after H2O2 treatment. Our data revealed redox-sensitive events in translation, growth signaling, and stress response pathways, and identified redox-regulated cysteines that are important for signaling through the p38 MAP kinase (MAPK) pathway. Our in-depth proteomic dataset provides a molecular basis for understanding redox signaling in vivo, and will serve as a valuable and rich resource for the field of redox biology.

Suggested Citation

  • Jin Meng & Ling Fu & Keke Liu & Caiping Tian & Ziyun Wu & Youngeun Jung & Renan B. Ferreira & Kate S. Carroll & T. Keith Blackwell & Jing Yang, 2021. "Global profiling of distinct cysteine redox forms reveals wide-ranging redox regulation in C. elegans," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-21686-3
    DOI: 10.1038/s41467-021-21686-3
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-021-21686-3
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-021-21686-3?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Renan B. Ferreira & Ling Fu & Youngeun Jung & Jing Yang & Kate S. Carroll, 2022. "Reaction-based fluorogenic probes for detecting protein cysteine oxidation in living cells," Nature Communications, Nature, vol. 13(1), pages 1-12, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-21686-3. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.