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Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP

Author

Listed:
  • Lukas Bokelmann

    (Max Planck Institute for Evolutionary Anthropology)

  • Olaf Nickel

    (Hospital St. Georg)

  • Tomislav Maricic

    (Max Planck Institute for Evolutionary Anthropology)

  • Svante Pääbo

    (Max Planck Institute for Evolutionary Anthropology
    Okinawa Institute of Science and Technology)

  • Matthias Meyer

    (Max Planck Institute for Evolutionary Anthropology)

  • Stephan Borte

    (Hospital St. Georg
    ImmunoDeficiency Center Leipzig (IDCL) at Hospital St. Georg Leipzig, Jeffrey Modell Diagnostic and Research Center for Primary Immunodeficiency Diseases
    Karolinska Institutet at Karolinska University Hospital Huddinge)

  • Stephan Riesenberg

    (Max Planck Institute for Evolutionary Anthropology)

Abstract

Efforts to contain the spread of SARS-CoV-2 have spurred the need for reliable, rapid, and cost-effective diagnostic methods which can be applied to large numbers of people. However, current standard protocols for the detection of viral nucleic acids while sensitive, require a high level of automation and sophisticated laboratory equipment to achieve throughputs that allow whole communities to be tested on a regular basis. Here we present Cap-iLAMP (capture and improved loop-mediated isothermal amplification) which combines a hybridization capture-based RNA extraction of gargle lavage samples with an improved colorimetric RT-LAMP assay and smartphone-based color scoring. Cap-iLAMP is compatible with point-of-care testing and enables the detection of SARS-CoV-2 positive samples in less than one hour. In contrast to direct addition of the sample to improved LAMP (iLAMP), Cap-iLAMP prevents false positives and allows single positive samples to be detected in pools of 25 negative samples, reducing the reagent cost per test to ~1 Euro per individual.

Suggested Citation

  • Lukas Bokelmann & Olaf Nickel & Tomislav Maricic & Svante Pääbo & Matthias Meyer & Stephan Borte & Stephan Riesenberg, 2021. "Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP," Nature Communications, Nature, vol. 12(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-21627-0
    DOI: 10.1038/s41467-021-21627-0
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