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Exon junction complex dependent mRNA localization is linked to centrosome organization during ciliogenesis

Author

Listed:
  • Oh Sung Kwon

    (PSL Research University)

  • Rahul Mishra

    (PSL Research University
    Czech Academy of Sciences)

  • Adham Safieddine

    (CNRS
    CNRS)

  • Emeline Coleno

    (CNRS
    CNRS)

  • Quentin Alasseur

    (PSL Research University)

  • Marion Faucourt

    (PSL Research University)

  • Isabelle Barbosa

    (PSL Research University)

  • Edouard Bertrand

    (CNRS
    CNRS)

  • Nathalie Spassky

    (PSL Research University)

  • Hervé Le Hir

    (PSL Research University)

Abstract

Exon junction complexes (EJCs) mark untranslated spliced mRNAs and are crucial for the mRNA lifecycle. An imbalance in EJC dosage alters mouse neural stem cell (mNSC) division and is linked to human neurodevelopmental disorders. In quiescent mNSC and immortalized human retinal pigment epithelial (RPE1) cells, centrioles form a basal body for ciliogenesis. Here, we report that EJCs accumulate at basal bodies of mNSC or RPE1 cells and decline when these cells differentiate or resume growth. A high-throughput smFISH screen identifies two transcripts accumulating at centrosomes in quiescent cells, NIN and BICD2. In contrast to BICD2, the localization of NIN transcripts is EJC-dependent. NIN mRNA encodes a core component of centrosomes required for microtubule nucleation and anchoring. We find that EJC down-regulation impairs both pericentriolar material organization and ciliogenesis. An EJC-dependent mRNA trafficking towards centrosome and basal bodies might contribute to proper mNSC division and brain development.

Suggested Citation

  • Oh Sung Kwon & Rahul Mishra & Adham Safieddine & Emeline Coleno & Quentin Alasseur & Marion Faucourt & Isabelle Barbosa & Edouard Bertrand & Nathalie Spassky & Hervé Le Hir, 2021. "Exon junction complex dependent mRNA localization is linked to centrosome organization during ciliogenesis," Nature Communications, Nature, vol. 12(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-21590-w
    DOI: 10.1038/s41467-021-21590-w
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    Cited by:

    1. Olivier Bensaude & Isabelle Barbosa & Lucia Morillo & Rivka Dikstein & Hervé Hir, 2024. "Exon-junction complex association with stalled ribosomes and slow translation-independent disassembly," Nature Communications, Nature, vol. 15(1), pages 1-14, December.

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