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A choreography of centrosomal mRNAs reveals a conserved localization mechanism involving active polysome transport

Author

Listed:
  • Adham Safieddine

    (University of Montpellier, CNRS
    University of Montpellier, CNRS
    Lebanese University)

  • Emeline Coleno

    (University of Montpellier, CNRS
    University of Montpellier, CNRS)

  • Soha Salloum

    (University of Montpellier, CNRS
    University of Montpellier, CNRS
    Lebanese University)

  • Arthur Imbert

    (MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology
    Institut Curie
    INSERM, U900)

  • Abdel-Meneem Traboulsi

    (University of Montpellier, CNRS
    University of Montpellier, CNRS)

  • Oh Sung Kwon

    (PSL Research University)

  • Frederic Lionneton

    (BioCampus Montpellier)

  • Virginie Georget

    (BioCampus Montpellier)

  • Marie-Cécile Robert

    (University of Montpellier, CNRS
    University of Montpellier, CNRS)

  • Thierry Gostan

    (University of Montpellier, CNRS)

  • Charles-Henri Lecellier

    (University of Montpellier, CNRS
    University of Montpellier, CNRS)

  • Racha Chouaib

    (University of Montpellier, CNRS
    University of Montpellier, CNRS
    Lebanese University)

  • Xavier Pichon

    (University of Montpellier, CNRS
    University of Montpellier, CNRS)

  • Hervé Hir

    (PSL Research University)

  • Kazem Zibara

    (Lebanese University)

  • Florian Mueller

    (Imaging and Modeling Unit, Institut Pasteur, UMR 3691 CNRS, C3BI USR 3756 IP CNRS)

  • Thomas Walter

    (MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology
    Institut Curie
    INSERM, U900)

  • Marion Peter

    (University of Montpellier, CNRS
    University of Montpellier, CNRS)

  • Edouard Bertrand

    (University of Montpellier, CNRS
    University of Montpellier, CNRS
    University of Montpellier, CNRS)

Abstract

Local translation allows for a spatial control of gene expression. Here, we use high-throughput smFISH to screen centrosomal protein-coding genes, and we describe 8 human mRNAs accumulating at centrosomes. These mRNAs localize at different stages during cell cycle with a remarkable choreography, indicating a finely regulated translational program at centrosomes. Interestingly, drug treatments and reporter analyses reveal a common translation-dependent localization mechanism requiring the nascent protein. Using ASPM and NUMA1 as models, single mRNA and polysome imaging reveals active movements of endogenous polysomes towards the centrosome at the onset of mitosis, when these mRNAs start localizing. ASPM polysomes associate with microtubules and localize by either motor-driven transport or microtubule pulling. Remarkably, the Drosophila orthologs of the human centrosomal mRNAs also localize to centrosomes and also require translation. These data identify a conserved family of centrosomal mRNAs that localize by active polysome transport mediated by nascent proteins.

Suggested Citation

  • Adham Safieddine & Emeline Coleno & Soha Salloum & Arthur Imbert & Abdel-Meneem Traboulsi & Oh Sung Kwon & Frederic Lionneton & Virginie Georget & Marie-Cécile Robert & Thierry Gostan & Charles-Henri , 2021. "A choreography of centrosomal mRNAs reveals a conserved localization mechanism involving active polysome transport," Nature Communications, Nature, vol. 12(1), pages 1-21, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-21585-7
    DOI: 10.1038/s41467-021-21585-7
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