IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v12y2021i1d10.1038_s41467-021-21557-x.html
   My bibliography  Save this article

Blastocyst complementation using Prdm14-deficient rats enables efficient germline transmission and generation of functional mouse spermatids in rats

Author

Listed:
  • Toshihiro Kobayashi

    (National Institute for Physiological Sciences
    The Graduate University of Advanced Studies)

  • Teppei Goto

    (National Institute for Physiological Sciences)

  • Mami Oikawa

    (National Institute for Physiological Sciences)

  • Makoto Sanbo

    (National Institute for Physiological Sciences)

  • Fumika Yoshida

    (National Institute for Physiological Sciences)

  • Reiko Terada

    (National Institute for Physiological Sciences)

  • Naoko Niizeki

    (National Institute for Physiological Sciences)

  • Naoyo Kajitani

    (Tottori University)

  • Kanako Kazuki

    (Tottori University)

  • Yasuhiro Kazuki

    (Tottori University
    Tottori University)

  • Shinichi Hochi

    (Shinshu University)

  • Hiromitsu Nakauchi

    (The University of Tokyo
    Stanford University School of Medicine)

  • M. Azim Surani

    (University of Cambridge
    University of Cambridge)

  • Masumi Hirabayashi

    (National Institute for Physiological Sciences
    The Graduate University of Advanced Studies)

Abstract

Murine animal models from genetically modified pluripotent stem cells (PSCs) are essential for functional genomics and biomedical research, which require germline transmission for the establishment of colonies. However, the quality of PSCs, and donor-host cell competition in chimeras often present strong barriers for germline transmission. Here, we report efficient germline transmission of recalcitrant PSCs via blastocyst complementation, a method to compensate for missing tissues or organs in genetically modified animals via blastocyst injection of PSCs. We show that blastocysts from germline-deficient Prdm14 knockout rats provide a niche for the development of gametes originating entirely from the donor PSCs without any detriment to somatic development. We demonstrate the potential of this approach by creating PSC-derived Pax2/Pax8 double mutant anephric rats, and rescuing germline transmission of a PSC carrying a mouse artificial chromosome. Furthermore, we generate mouse PSC-derived functional spermatids in rats, which provides a proof-of-principle for the generation of xenogenic gametes in vivo. We believe this approach will become a useful system for generating PSC-derived germ cells in the future.

Suggested Citation

  • Toshihiro Kobayashi & Teppei Goto & Mami Oikawa & Makoto Sanbo & Fumika Yoshida & Reiko Terada & Naoko Niizeki & Naoyo Kajitani & Kanako Kazuki & Yasuhiro Kazuki & Shinichi Hochi & Hiromitsu Nakauchi , 2021. "Blastocyst complementation using Prdm14-deficient rats enables efficient germline transmission and generation of functional mouse spermatids in rats," Nature Communications, Nature, vol. 12(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-21557-x
    DOI: 10.1038/s41467-021-21557-x
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-021-21557-x
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-021-21557-x?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Hisato Nagano & Naoaki Mizuno & Hideyuki Sato & Eiji Mizutani & Ayaka Yanagida & Mayuko Kano & Mariko Kasai & Hiromi Yamamoto & Motoo Watanabe & Fabian Suchy & Hideki Masaki & Hiromitsu Nakauchi, 2024. "Skin graft with dermis and appendages generated in vivo by cell competition," Nature Communications, Nature, vol. 15(1), pages 1-12, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-21557-x. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.