IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v12y2021i1d10.1038_s41467-021-21529-1.html
   My bibliography  Save this article

Interaction of 7SK with the Smn complex modulates snRNP production

Author

Listed:
  • Changhe Ji

    (University Hospital Wuerzburg)

  • Jakob Bader

    (Max Planck Institute of Biochemistry)

  • Pradhipa Ramanathan

    (University of Wuerzburg)

  • Luisa Hennlein

    (University Hospital Wuerzburg)

  • Felix Meissner

    (Max Planck Institute of Biochemistry
    Max Planck Institute of Biochemistry
    University of Bonn)

  • Sibylle Jablonka

    (University Hospital Wuerzburg)

  • Matthias Mann

    (Max Planck Institute of Biochemistry
    University of Copenhagen)

  • Utz Fischer

    (University of Wuerzburg)

  • Michael Sendtner

    (University Hospital Wuerzburg)

  • Michael Briese

    (University Hospital Wuerzburg)

Abstract

Gene expression requires tight coordination of the molecular machineries that mediate transcription and splicing. While the interplay between transcription kinetics and spliceosome fidelity has been investigated before, less is known about mechanisms regulating the assembly of the spliceosomal machinery in response to transcription changes. Here, we report an association of the Smn complex, which mediates spliceosomal snRNP biogenesis, with the 7SK complex involved in transcriptional regulation. We found that Smn interacts with the 7SK core components Larp7 and Mepce and specifically associates with 7SK subcomplexes containing hnRNP R. The association between Smn and 7SK complexes is enhanced upon transcriptional inhibition leading to reduced production of snRNPs. Taken together, our findings reveal a functional association of Smn and 7SK complexes that is governed by global changes in transcription. Thus, in addition to its canonical nuclear role in transcriptional regulation, 7SK has cytosolic functions in fine-tuning spliceosome production according to transcriptional demand.

Suggested Citation

  • Changhe Ji & Jakob Bader & Pradhipa Ramanathan & Luisa Hennlein & Felix Meissner & Sibylle Jablonka & Matthias Mann & Utz Fischer & Michael Sendtner & Michael Briese, 2021. "Interaction of 7SK with the Smn complex modulates snRNP production," Nature Communications, Nature, vol. 12(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-21529-1
    DOI: 10.1038/s41467-021-21529-1
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-021-21529-1
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-021-21529-1?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Saeede Salehi & Abdolhossein Zare & Gianluca Prezza & Jakob Bader & Cornelius Schneider & Utz Fischer & Felix Meissner & Matthias Mann & Michael Briese & Michael Sendtner, 2023. "Cytosolic Ptbp2 modulates axon growth in motoneurons through axonal localization and translation of Hnrnpr," Nature Communications, Nature, vol. 14(1), pages 1-19, December.
    2. Diego J. Páez-Moscoso & David V. Ho & Lili Pan & Katie Hildebrand & Kristi L. Jensen & Michaella J. Levy & Laurence Florens & Peter Baumann, 2022. "A putative cap binding protein and the methyl phosphate capping enzyme Bin3/MePCE function in telomerase biogenesis," Nature Communications, Nature, vol. 13(1), pages 1-16, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-21529-1. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.