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Unravelling the structural complexity of glycolipids with cryogenic infrared spectroscopy

Author

Listed:
  • Carla Kirschbaum

    (Institut für Chemie und Biochemie, Freie Universität Berlin
    Fritz-Haber-Institut der Max-Planck-Gesellschaft)

  • Kim Greis

    (Institut für Chemie und Biochemie, Freie Universität Berlin
    Fritz-Haber-Institut der Max-Planck-Gesellschaft)

  • Eike Mucha

    (Fritz-Haber-Institut der Max-Planck-Gesellschaft)

  • Lisa Kain

    (Scripps Research)

  • Shenglou Deng

    (Brigham Young University)

  • Andreas Zappe

    (Institut für Chemie und Biochemie, Freie Universität Berlin)

  • Sandy Gewinner

    (Fritz-Haber-Institut der Max-Planck-Gesellschaft)

  • Wieland Schöllkopf

    (Fritz-Haber-Institut der Max-Planck-Gesellschaft)

  • Gert Helden

    (Fritz-Haber-Institut der Max-Planck-Gesellschaft)

  • Gerard Meijer

    (Fritz-Haber-Institut der Max-Planck-Gesellschaft)

  • Paul B. Savage

    (Brigham Young University)

  • Mateusz Marianski

    (The City University of New York
    The City University of New York)

  • Luc Teyton

    (Scripps Research)

  • Kevin Pagel

    (Institut für Chemie und Biochemie, Freie Universität Berlin
    Fritz-Haber-Institut der Max-Planck-Gesellschaft)

Abstract

Glycolipids are complex glycoconjugates composed of a glycan headgroup and a lipid moiety. Their modular biosynthesis creates a vast amount of diverse and often isomeric structures, which fulfill highly specific biological functions. To date, no gold-standard analytical technique can provide a comprehensive structural elucidation of complex glycolipids, and insufficient tools for isomer distinction can lead to wrong assignments. Herein we use cryogenic gas-phase infrared spectroscopy to systematically investigate different kinds of isomerism in immunologically relevant glycolipids. We show that all structural features, including isomeric glycan headgroups, anomeric configurations and different lipid moieties, can be unambiguously resolved by diagnostic spectroscopic fingerprints in a narrow spectral range. The results allow for the characterization of isomeric glycolipid mixtures and biological applications.

Suggested Citation

  • Carla Kirschbaum & Kim Greis & Eike Mucha & Lisa Kain & Shenglou Deng & Andreas Zappe & Sandy Gewinner & Wieland Schöllkopf & Gert Helden & Gerard Meijer & Paul B. Savage & Mateusz Marianski & Luc Tey, 2021. "Unravelling the structural complexity of glycolipids with cryogenic infrared spectroscopy," Nature Communications, Nature, vol. 12(1), pages 1-7, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-21480-1
    DOI: 10.1038/s41467-021-21480-1
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    Cited by:

    1. Jua Lee & Dongtan Yin & Jaekyung Yun & Minsoo Kim & Seong-Wook Kim & Heeyoun Hwang & Ji Eun Park & Boyoung Lee & C. Justin Lee & Hee-Sup Shin & Hyun Joo An, 2024. "Deciphering mouse brain spatial diversity via glyco-lipidomic mapping," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

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