IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v12y2021i1d10.1038_s41467-021-21373-3.html
   My bibliography  Save this article

Acoustofluidic rotational tweezing enables high-speed contactless morphological phenotyping of zebrafish larvae

Author

Listed:
  • Chuyi Chen

    (Duke University)

  • Yuyang Gu

    (Duke University)

  • Julien Philippe

    (Duke University Medical Center)

  • Peiran Zhang

    (Duke University)

  • Hunter Bachman

    (Duke University)

  • Jinxin Zhang

    (Duke University)

  • John Mai

    (Alfred E. Mann Institute for Biomedical Engineering, University of Southern California)

  • Joseph Rufo

    (Duke University)

  • John F. Rawls

    (Duke University)

  • Erica E. Davis

    (Duke University Medical Center
    Stanley Manne Children’s Research Institute, Ann & Robert H. Lurie Children’s Hospital of Chicago
    Northwestern University)

  • Nicholas Katsanis

    (Duke University Medical Center
    Stanley Manne Children’s Research Institute, Ann & Robert H. Lurie Children’s Hospital of Chicago
    Northwestern University)

  • Tony Jun Huang

    (Duke University)

Abstract

Modern biomedical research and preclinical pharmaceutical development rely heavily on the phenotyping of small vertebrate models for various diseases prior to human testing. In this article, we demonstrate an acoustofluidic rotational tweezing platform that enables contactless, high-speed, 3D multispectral imaging and digital reconstruction of zebrafish larvae for quantitative phenotypic analysis. The acoustic-induced polarized vortex streaming achieves contactless and rapid (~1 s/rotation) rotation of zebrafish larvae. This enables multispectral imaging of the zebrafish body and internal organs from different viewing perspectives. Moreover, we develop a 3D reconstruction pipeline that yields accurate 3D models based on the multi-view images for quantitative evaluation of basic morphological characteristics and advanced combinations of metrics. With its contactless nature and advantages in speed and automation, our acoustofluidic rotational tweezing system has the potential to be a valuable asset in numerous fields, especially for developmental biology, small molecule screening in biochemistry, and pre-clinical drug development in pharmacology.

Suggested Citation

  • Chuyi Chen & Yuyang Gu & Julien Philippe & Peiran Zhang & Hunter Bachman & Jinxin Zhang & John Mai & Joseph Rufo & John F. Rawls & Erica E. Davis & Nicholas Katsanis & Tony Jun Huang, 2021. "Acoustofluidic rotational tweezing enables high-speed contactless morphological phenotyping of zebrafish larvae," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-21373-3
    DOI: 10.1038/s41467-021-21373-3
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-021-21373-3
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-021-21373-3?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Jan Durrer & Prajwal Agrawal & Ali Ozgul & Stephan C. F. Neuhauss & Nitesh Nama & Daniel Ahmed, 2022. "A robot-assisted acoustofluidic end effector," Nature Communications, Nature, vol. 13(1), pages 1-13, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-21373-3. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.