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Acoustofluidic rotational tweezing enables high-speed contactless morphological phenotyping of zebrafish larvae

Author

Listed:
  • Chuyi Chen

    (Duke University)

  • Yuyang Gu

    (Duke University)

  • Julien Philippe

    (Duke University Medical Center)

  • Peiran Zhang

    (Duke University)

  • Hunter Bachman

    (Duke University)

  • Jinxin Zhang

    (Duke University)

  • John Mai

    (Alfred E. Mann Institute for Biomedical Engineering, University of Southern California)

  • Joseph Rufo

    (Duke University)

  • John F. Rawls

    (Duke University)

  • Erica E. Davis

    (Duke University Medical Center
    Stanley Manne Children’s Research Institute, Ann & Robert H. Lurie Children’s Hospital of Chicago
    Northwestern University)

  • Nicholas Katsanis

    (Duke University Medical Center
    Stanley Manne Children’s Research Institute, Ann & Robert H. Lurie Children’s Hospital of Chicago
    Northwestern University)

  • Tony Jun Huang

    (Duke University)

Abstract

Modern biomedical research and preclinical pharmaceutical development rely heavily on the phenotyping of small vertebrate models for various diseases prior to human testing. In this article, we demonstrate an acoustofluidic rotational tweezing platform that enables contactless, high-speed, 3D multispectral imaging and digital reconstruction of zebrafish larvae for quantitative phenotypic analysis. The acoustic-induced polarized vortex streaming achieves contactless and rapid (~1 s/rotation) rotation of zebrafish larvae. This enables multispectral imaging of the zebrafish body and internal organs from different viewing perspectives. Moreover, we develop a 3D reconstruction pipeline that yields accurate 3D models based on the multi-view images for quantitative evaluation of basic morphological characteristics and advanced combinations of metrics. With its contactless nature and advantages in speed and automation, our acoustofluidic rotational tweezing system has the potential to be a valuable asset in numerous fields, especially for developmental biology, small molecule screening in biochemistry, and pre-clinical drug development in pharmacology.

Suggested Citation

  • Chuyi Chen & Yuyang Gu & Julien Philippe & Peiran Zhang & Hunter Bachman & Jinxin Zhang & John Mai & Joseph Rufo & John F. Rawls & Erica E. Davis & Nicholas Katsanis & Tony Jun Huang, 2021. "Acoustofluidic rotational tweezing enables high-speed contactless morphological phenotyping of zebrafish larvae," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-21373-3
    DOI: 10.1038/s41467-021-21373-3
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    Cited by:

    1. Liang Shen & Zhenhua Tian & Kaichun Yang & Joseph Rich & Jianping Xia & Neil Upreti & Jinxin Zhang & Chuyi Chen & Nanjing Hao & Zhichao Pei & Tony Jun Huang, 2024. "Joint subarray acoustic tweezers enable controllable cell translation, rotation, and deformation," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    2. Jan Durrer & Prajwal Agrawal & Ali Ozgul & Stephan C. F. Neuhauss & Nitesh Nama & Daniel Ahmed, 2022. "A robot-assisted acoustofluidic end effector," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    3. Peng Pan & Michael Zoberman & Pengsong Zhang & Sharanja Premachandran & Sanjana Bhatnagar & Pallavi P. Pilaka-Akella & William Sun & Chengyin Li & Charlotte Martin & Pengfei Xu & Zefang Zhang & Ryan L, 2024. "Robotic microinjection enables large-scale transgenic studies of Caenorhabditis elegans," Nature Communications, Nature, vol. 15(1), pages 1-15, December.

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