IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v12y2021i1d10.1038_s41467-021-21344-8.html
   My bibliography  Save this article

20S proteasomes secreted by the malaria parasite promote its growth

Author

Listed:
  • Elya Dekel

    (Weizmann Institute of Science)

  • Dana Yaffe

    (Weizmann Institute of Science)

  • Irit Rosenhek-Goldian

    (Weizmann Institute of Science)

  • Gili Ben-Nissan

    (Weizmann Institute of Science)

  • Yifat Ofir-Birin

    (Weizmann Institute of Science)

  • Mattia I. Morandi

    (Weizmann Institute of Science)

  • Tamar Ziv

    (Technion - Israel Institute of Technology)

  • Xavier Sisquella

    (The Walter and Eliza Hall Institute of Medical Research
    The University of Melbourne)

  • Matthew A. Pimentel

    (The Walter and Eliza Hall Institute of Medical Research
    The University of Melbourne)

  • Thomas Nebl

    (The Walter and Eliza Hall Institute of Medical Research
    The University of Melbourne)

  • Eugene Kapp

    (The Walter and Eliza Hall Institute of Medical Research
    The University of Melbourne)

  • Yael Ohana Daniel

    (Weizmann Institute of Science)

  • Paula Abou Karam

    (Weizmann Institute of Science)

  • Daniel Alfandari

    (Weizmann Institute of Science)

  • Ron Rotkopf

    (Weizmann Institute of Science)

  • Shimrit Malihi

    (Weizmann Institute of Science)

  • Tal Block Temin

    (Weizmann Institute of Science)

  • Debakshi Mullick

    (Weizmann Institute of Science)

  • Or-Yam Revach

    (Weizmann Institute of Science)

  • Ariel Rudik

    (Weizmann Institute of Science)

  • Nir S. Gov

    (Weizmann Institute of Science)

  • Ido Azuri

    (Weizmann Institute of Science)

  • Ziv Porat

    (Flow Cytometry Unit, Life Sciences Core Facilities, Weizmann Institute of Science)

  • Giulia Bergamaschi

    (Vrije Universiteit Amsterdam)

  • Raya Sorkin

    (Tel Aviv University
    Tel Aviv University)

  • Gijs J. L. Wuite

    (Vrije Universiteit Amsterdam)

  • Ori Avinoam

    (Weizmann Institute of Science)

  • Teresa G. Carvalho

    (La Trobe University)

  • Sidney R. Cohen

    (Weizmann Institute of Science)

  • Michal Sharon

    (Weizmann Institute of Science)

  • Neta Regev-Rudzki

    (Weizmann Institute of Science)

Abstract

Mature red blood cells (RBCs) lack internal organelles and canonical defense mechanisms, making them both a fascinating host cell, in general, and an intriguing choice for the deadly malaria parasite Plasmodium falciparum (Pf), in particular. Pf, while growing inside its natural host, the human RBC, secretes multipurpose extracellular vesicles (EVs), yet their influence on this essential host cell remains unknown. Here we demonstrate that Pf parasites, cultured in fresh human donor blood, secrete within such EVs assembled and functional 20S proteasome complexes (EV-20S). The EV-20S proteasomes modulate the mechanical properties of naïve human RBCs by remodeling their cytoskeletal network. Furthermore, we identify four degradation targets of the secreted 20S proteasome, the phosphorylated cytoskeletal proteins β-adducin, ankyrin-1, dematin and Epb4.1. Overall, our findings reveal a previously unknown 20S proteasome secretion mechanism employed by the human malaria parasite, which primes RBCs for parasite invasion by altering membrane stiffness, to facilitate malaria parasite growth.

Suggested Citation

  • Elya Dekel & Dana Yaffe & Irit Rosenhek-Goldian & Gili Ben-Nissan & Yifat Ofir-Birin & Mattia I. Morandi & Tamar Ziv & Xavier Sisquella & Matthew A. Pimentel & Thomas Nebl & Eugene Kapp & Yael Ohana D, 2021. "20S proteasomes secreted by the malaria parasite promote its growth," Nature Communications, Nature, vol. 12(1), pages 1-19, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-21344-8
    DOI: 10.1038/s41467-021-21344-8
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-021-21344-8
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-021-21344-8?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-21344-8. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.