IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v12y2021i1d10.1038_s41467-021-21251-y.html
   My bibliography  Save this article

Complete and cooperative in vitro assembly of computationally designed self-assembling protein nanomaterials

Author

Listed:
  • Adam J. Wargacki

    (University of Washington
    University of Washington)

  • Tobias P. Wörner

    (Utrecht University)

  • Michiel Waterbeemd

    (Utrecht University)

  • Daniel Ellis

    (University of Washington
    University of Washington
    University of Washington)

  • Albert J. R. Heck

    (Utrecht University)

  • Neil P. King

    (University of Washington
    University of Washington)

Abstract

Recent advances in computational methods have enabled the predictive design of self-assembling protein nanomaterials with atomic-level accuracy. These design strategies focus exclusively on a single target structure, without consideration of the mechanism or dynamics of assembly. However, understanding the assembly process, and in particular its robustness to perturbation, will be critical for translating this class of materials into useful technologies. Here we investigate the assembly of two computationally designed, 120-subunit icosahedral complexes in detail using several complementary biochemical methods. We found that assembly of each material from its two constituent protein building blocks was highly cooperative and yielded exclusively complete, 120-subunit complexes except in one non-stoichiometric regime for one of the materials. Our results suggest that in vitro assembly provides a robust and controllable route for the manufacture of designed protein nanomaterials and confirm that cooperative assembly can be an intrinsic, rather than evolved, feature of hierarchically structured protein complexes.

Suggested Citation

  • Adam J. Wargacki & Tobias P. Wörner & Michiel Waterbeemd & Daniel Ellis & Albert J. R. Heck & Neil P. King, 2021. "Complete and cooperative in vitro assembly of computationally designed self-assembling protein nanomaterials," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-21251-y
    DOI: 10.1038/s41467-021-21251-y
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-021-21251-y
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-021-21251-y?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Geoffrey B. Hutchinson & Olubukola M. Abiona & Cynthia T. Ziwawo & Anne P. Werner & Daniel Ellis & Yaroslav Tsybovsky & Sarah R. Leist & Charis Palandjian & Ande West & Ethan J. Fritch & Nianshuang Wa, 2023. "Nanoparticle display of prefusion coronavirus spike elicits S1-focused cross-reactive antibody response against diverse coronavirus subgenera," Nature Communications, Nature, vol. 14(1), pages 1-11, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-21251-y. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.