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Cryo-EM structures of engineered active bc1-cbb3 type CIII2CIV super-complexes and electronic communication between the complexes

Author

Listed:
  • Stefan Steimle

    (University of Pennsylvania)

  • Trevor van Eeuwen

    (Perelman School of Medicine, University of Pennsylvania)

  • Yavuz Ozturk

    (University of Pennsylvania
    Albert-Ludwigs University of Freiburg)

  • Hee Jong Kim

    (Perelman School of Medicine, University of Pennsylvania)

  • Merav Braitbard

    (Institute of Life Sciences, The Hebrew University of Jerusalem)

  • Nur Selamoglu

    (University of Pennsylvania)

  • Benjamin A. Garcia

    (Perelman School of Medicine, University of Pennsylvania)

  • Dina Schneidman-Duhovny

    (Institute of Life Sciences, The Hebrew University of Jerusalem)

  • Kenji Murakami

    (Perelman School of Medicine, University of Pennsylvania)

  • Fevzi Daldal

    (University of Pennsylvania)

Abstract

Respiratory electron transport complexes are organized as individual entities or combined as large supercomplexes (SC). Gram-negative bacteria deploy a mitochondrial-like cytochrome (cyt) bc1 (Complex III, CIII2), and may have specific cbb3-type cyt c oxidases (Complex IV, CIV) instead of the canonical aa3-type CIV. Electron transfer between these complexes is mediated by soluble (c2) and membrane-anchored (cy) cyts. Here, we report the structure of an engineered bc1-cbb3 type SC (CIII2CIV, 5.2 Å resolution) and three conformers of native CIII2 (3.3 Å resolution). The SC is active in vivo and in vitro, contains all catalytic subunits and cofactors, and two extra transmembrane helices attributed to cyt cy and the assembly factor CcoH. The cyt cy is integral to SC, its cyt domain is mobile and it conveys electrons to CIV differently than cyt c2. The successful production of a native-like functional SC and determination of its structure illustrate the characteristics of membrane-confined and membrane-external respiratory electron transport pathways in Gram-negative bacteria.

Suggested Citation

  • Stefan Steimle & Trevor van Eeuwen & Yavuz Ozturk & Hee Jong Kim & Merav Braitbard & Nur Selamoglu & Benjamin A. Garcia & Dina Schneidman-Duhovny & Kenji Murakami & Fevzi Daldal, 2021. "Cryo-EM structures of engineered active bc1-cbb3 type CIII2CIV super-complexes and electronic communication between the complexes," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-21051-4
    DOI: 10.1038/s41467-021-21051-4
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