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A multiplex chemiluminescent immunoassay for serological profiling of COVID-19-positive symptomatic and asymptomatic patients

Author

Listed:
  • Allison N. Grossberg

    (Knoebel Institute for Healthy Aging, University of Denver
    University of Denver)

  • Lilia A. Koza

    (Knoebel Institute for Healthy Aging, University of Denver
    University of Denver)

  • Aurélie Ledreux

    (Knoebel Institute for Healthy Aging, University of Denver)

  • Chad Prusmack

    (Resilience Code)

  • Hari Krishnan Krishnamurthy

    (Vibrant Sciences LLC)

  • Vasanth Jayaraman

    (Vibrant Sciences LLC)

  • Ann-Charlotte Granholm

    (Knoebel Institute for Healthy Aging, University of Denver)

  • Daniel A. Linseman

    (Knoebel Institute for Healthy Aging, University of Denver
    University of Denver
    Eleanor Roosevelt Institute, University of Denver)

Abstract

The COVID-19 pandemic affects more than 81 million people worldwide with over 1.7 million deaths. As the population returns to work, it is critical to develop tests that reliably detect SARS-CoV-2-specific antibodies. Here we present results from a multiplex serology test for assessing the antibody responses to COVID-19. In an initial large cohort, this test shows greater than 99% agreement with COVID-19 PCR test. In a second outpatient cohort consisting of adults and children in Colorado, the IgG responses are more robust in positive/symptomatic participants than in positive/asymptomatic participants, the IgM responses in symptomatic participants are transient and largely fall below the detection limit 30 days after symptom onset, and the levels of IgA against SARS-CoV-2 receptor binding domain are significantly increased in participants with moderate-to-severe symptoms compared to those with mild-to-moderate symptoms or asymptomatic individuals. Our results thus provide insight into serology profiling and the immune response to COVID-19.

Suggested Citation

  • Allison N. Grossberg & Lilia A. Koza & Aurélie Ledreux & Chad Prusmack & Hari Krishnan Krishnamurthy & Vasanth Jayaraman & Ann-Charlotte Granholm & Daniel A. Linseman, 2021. "A multiplex chemiluminescent immunoassay for serological profiling of COVID-19-positive symptomatic and asymptomatic patients," Nature Communications, Nature, vol. 12(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-21040-7
    DOI: 10.1038/s41467-021-21040-7
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