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LEAFY is a pioneer transcription factor and licenses cell reprogramming to floral fate

Author

Listed:
  • Run Jin

    (University of Pennsylvania)

  • Samantha Klasfeld

    (University of Pennsylvania)

  • Yang Zhu

    (University of Pennsylvania)

  • Meilin Fernandez Garcia

    (School of Medicine
    Icahn School of Medicine at Mount Sinai)

  • Jun Xiao

    (University of Pennsylvania
    Chinese Academy of Sciences
    The John Innes Centre, Norwich Research Park)

  • Soon-Ki Han

    (University of Pennsylvania
    Nagoya University)

  • Adam Konkol

    (University of Pennsylvania)

  • Doris Wagner

    (University of Pennsylvania)

Abstract

Master transcription factors reprogram cell fate in multicellular eukaryotes. Pioneer transcription factors have prominent roles in this process because of their ability to contact their cognate binding motifs in closed chromatin. Reprogramming is pervasive in plants, whose development is plastic and tuned by the environment, yet little is known about pioneer transcription factors in this kingdom. Here, we show that the master transcription factor LEAFY (LFY), which promotes floral fate through upregulation of the floral commitment factor APETALA1 (AP1), is a pioneer transcription factor. In vitro, LFY binds to the endogenous AP1 target locus DNA assembled into a nucleosome. In vivo, LFY associates with nucleosome occupied binding sites at the majority of its target loci, including AP1. Upon binding, LFY ‘unlocks’ chromatin locally by displacing the H1 linker histone and by recruiting SWI/SNF chromatin remodelers, but broad changes in chromatin accessibility occur later. Our study provides a mechanistic framework for patterning of inflorescence architecture and uncovers striking similarities between LFY and animal pioneer transcription factor.

Suggested Citation

  • Run Jin & Samantha Klasfeld & Yang Zhu & Meilin Fernandez Garcia & Jun Xiao & Soon-Ki Han & Adam Konkol & Doris Wagner, 2021. "LEAFY is a pioneer transcription factor and licenses cell reprogramming to floral fate," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-020-20883-w
    DOI: 10.1038/s41467-020-20883-w
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    Cited by:

    1. Jonathan Cahn & Michael Regulski & Jason Lynn & Evan Ernst & Cristiane Santis Alves & Srividya Ramakrishnan & Kapeel Chougule & Sharon Wei & Zhenyuan Lu & Xiaosa Xu & Umamaheswari Ramu & Jorg Drenkow , 2024. "MaizeCODE reveals bi-directionally expressed enhancers that harbor molecular signatures of maize domestication," Nature Communications, Nature, vol. 15(1), pages 1-17, December.

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