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STIM-Orai1 signaling regulates fluidity of cytoplasm during membrane blebbing

Author

Listed:
  • Kana Aoki

    (Kyushu University)

  • Shota Harada

    (Kyushu University)

  • Keita Kawaji

    (Kyushu University)

  • Kenji Matsuzawa

    (Kyushu University)

  • Seiichi Uchida

    (Kyushu University)

  • Junichi Ikenouchi

    (Kyushu University)

Abstract

The cytoplasm in mammalian cells is considered homogeneous. In this study, we report that the cytoplasmic fluidity is regulated in the blebbing cells; the cytoplasm of rapidly expanding membrane blebs is more disordered than the cytoplasm of retracting blebs. The increase of cytoplasmic fluidity in the expanding bleb is caused by a sharp rise in the calcium concentration. The STIM-Orai1 pathway regulates this rapid and restricted increase of calcium in the expanding blebs. Conversely, activated ERM protein binds to Orai1 to inhibit the store-operated calcium entry in retracting blebs, which results in decreased in cytoplasmic calcium, rapid reassembly of the actin cortex.

Suggested Citation

  • Kana Aoki & Shota Harada & Keita Kawaji & Kenji Matsuzawa & Seiichi Uchida & Junichi Ikenouchi, 2021. "STIM-Orai1 signaling regulates fluidity of cytoplasm during membrane blebbing," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-020-20826-5
    DOI: 10.1038/s41467-020-20826-5
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