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Simultaneous Zn2+ tracking in multiple organelles using super-resolution morphology-correlated organelle identification in living cells

Author

Listed:
  • Hongbao Fang

    (Nanjing University
    University of Cincinnati College of Medicine
    Nanjing University)

  • Shanshan Geng

    (Nanjing University)

  • Mingang Hao

    (University of Cincinnati College of Medicine)

  • Qixin Chen

    (University of Cincinnati College of Medicine)

  • Minglun Liu

    (Nanjing University)

  • Chunyan Liu

    (Cincinnati Children’s Hospital Medical Center)

  • Zhiqi Tian

    (University of Cincinnati College of Medicine)

  • Chengjun Wang

    (Sinopec Shengli Petroleum Engineering Limited Company)

  • Takanori Takebe

    (Cincinnati Children’s Hospital Medical Center
    Cincinnati Children’s Hospital Medical Center
    Cincinnati Children’s Hospital Medical Center
    University of Cincinnati College of Medicine)

  • Jun-Lin Guan

    (University of Cincinnati College of Medicine)

  • Yuncong Chen

    (Nanjing University
    Nanjing University)

  • Zijian Guo

    (Nanjing University
    Nanjing University)

  • Weijiang He

    (Nanjing University
    Nanjing University)

  • Jiajie Diao

    (University of Cincinnati College of Medicine)

Abstract

Zn2+ plays important roles in metabolism and signaling regulation. Subcellular Zn2+ compartmentalization is essential for organelle functions and cell biology, but there is currently no method to determine Zn2+ signaling relationships among more than two different organelles with one probe. Here, we report simultaneous Zn2+ tracking in multiple organelles (Zn-STIMO), a method that uses structured illumination microscopy (SIM) and a single Zn2+ fluorescent probe, allowing super-resolution morphology-correlated organelle identification in living cells. To guarantee SIM imaging quality for organelle identification, we develop a new turn-on Zn2+ fluorescent probe, NapBu-BPEA, by regulating the lipophilicity of naphthalimide-derived Zn2+ probes to make it accumulate in multiple organelles except the nucleus. Zn-STIMO with this probe shows that CCCP-induced mitophagy in HeLa cells is associated with labile Zn2+ enhancement. Therefore, direct organelle identification supported by SIM imaging makes Zn-STIMO a reliable method to determine labile Zn2+ dynamics in various organelles with one probe. Finally, SIM imaging of pluripotent stem cell-derived organoids with NapBu-BPEA demonstrates the potential of super-resolution morphology-correlated organelle identification to track biospecies and events in specific organelles within organoids.

Suggested Citation

  • Hongbao Fang & Shanshan Geng & Mingang Hao & Qixin Chen & Minglun Liu & Chunyan Liu & Zhiqi Tian & Chengjun Wang & Takanori Takebe & Jun-Lin Guan & Yuncong Chen & Zijian Guo & Weijiang He & Jiajie Dia, 2021. "Simultaneous Zn2+ tracking in multiple organelles using super-resolution morphology-correlated organelle identification in living cells," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-020-20309-7
    DOI: 10.1038/s41467-020-20309-7
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    Cited by:

    1. Sayaka Oda & Kazuhiro Nishiyama & Yuka Furumoto & Yohei Yamaguchi & Akiyuki Nishimura & Xiaokang Tang & Yuri Kato & Takuro Numaga-Tomita & Toshiyuki Kaneko & Supachoke Mangmool & Takuya Kuroda & Reish, 2022. "Myocardial TRPC6-mediated Zn2+ influx induces beneficial positive inotropy through β-adrenoceptors," Nature Communications, Nature, vol. 13(1), pages 1-16, December.

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