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Analysis of SARS-CoV-2 antibodies in COVID-19 convalescent blood using a coronavirus antigen microarray

Author

Listed:
  • Rafael R. Assis

    (University of California)

  • Aarti Jain

    (University of California)

  • Rie Nakajima

    (University of California)

  • Algis Jasinskas

    (University of California)

  • Jiin Felgner

    (University of California)

  • Joshua M. Obiero

    (University of California)

  • Philip J. Norris

    (Vitalant Research Institute
    University of California)

  • Mars Stone

    (Vitalant Research Institute
    University of California)

  • Graham Simmons

    (Vitalant Research Institute
    University of California)

  • Anil Bagri

    (Cerus Corporation)

  • Johannes Irsch

    (Cerus Corporation)

  • Martin Schreiber

    (Oregon Health & Science University)

  • Andreas Buser

    (University Hospital Basel, University of Basel)

  • Andreas Holbro

    (Oregon Health & Science University)

  • Manuel Battegay

    (University Hospital Basel, University of Basel)

  • Philip Hosimer

    (Ortho Clinical Diagnostics)

  • Charles Noesen

    (Ortho Clinical Diagnostics)

  • Oluwasanmi Adenaiye

    (University of Maryland)

  • Sheldon Tai

    (University of Maryland)

  • Filbert Hong

    (University of Maryland)

  • Donald K. Milton

    (University of Maryland)

  • D. Huw Davies

    (University of California)

  • Paul Contestable

    (Ortho Clinical Diagnostics)

  • Laurence M. Corash

    (Cerus Corporation)

  • Michael P. Busch

    (Vitalant Research Institute
    University of California)

  • Philip L. Felgner

    (University of California)

  • Saahir Khan

    (University of Southern California)

Abstract

The current practice for diagnosis of COVID-19, based on SARS-CoV-2 PCR testing of pharyngeal or respiratory specimens in a symptomatic patient at high epidemiologic risk, likely underestimates the true prevalence of infection. Serologic methods can more accurately estimate the disease burden by detecting infections missed by the limited testing performed to date. Here, we describe the validation of a coronavirus antigen microarray containing immunologically significant antigens from SARS-CoV-2, in addition to SARS-CoV, MERS-CoV, common human coronavirus strains, and other common respiratory viruses. A comparison of antibody profiles detected on the array from control sera collected prior to the SARS-CoV-2 pandemic versus convalescent blood specimens from virologically confirmed COVID-19 cases demonstrates near complete discrimination of these two groups, with improved performance from use of antigen combinations that include both spike protein and nucleoprotein. This array can be used as a diagnostic tool, as an epidemiologic tool to more accurately estimate the disease burden of COVID-19, and as a research tool to correlate antibody responses with clinical outcomes.

Suggested Citation

  • Rafael R. Assis & Aarti Jain & Rie Nakajima & Algis Jasinskas & Jiin Felgner & Joshua M. Obiero & Philip J. Norris & Mars Stone & Graham Simmons & Anil Bagri & Johannes Irsch & Martin Schreiber & Andr, 2021. "Analysis of SARS-CoV-2 antibodies in COVID-19 convalescent blood using a coronavirus antigen microarray," Nature Communications, Nature, vol. 12(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-020-20095-2
    DOI: 10.1038/s41467-020-20095-2
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