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PRC2 and EHMT1 regulate H3K27me2 and H3K27me3 establishment across the zygote genome

Author

Listed:
  • Tie-Gang Meng

    (Chinese Academy of Sciences
    Guangdong Second Provincial General Hospital)

  • Qian Zhou

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Xue-Shan Ma

    (The First Affiliated Hospital of Zhengzhou University, Zhengzhou)

  • Xiao-Yu Liu

    (Tongji University)

  • Qing-Ren Meng

    (Chinese Academy of Sciences)

  • Xian-Ju Huang

    (The First Affiliated Hospital of Zhengzhou University, Zhengzhou)

  • Hong-Lin Liu

    (Nanjing Agricultural University)

  • Wen-Long Lei

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Zheng-Hui Zhao

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Ying-Chun Ouyang

    (Chinese Academy of Sciences)

  • Yi Hou

    (Chinese Academy of Sciences)

  • Heide Schatten

    (University of Missouri)

  • Xiang-Hong Ou

    (Guangdong Second Provincial General Hospital)

  • Zhen-Bo Wang

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Shao-Rong Gao

    (Tongji University)

  • Qing-Yuan Sun

    (Chinese Academy of Sciences
    Guangdong Second Provincial General Hospital)

Abstract

The formation of zygote is the beginning of mammalian life, and dynamic epigenetic modifications are essential for mammalian normal development. H3K27 di-methylation (H3K27me2) and H3K27 tri-methylation (H3K27me3) are marks of facultative heterochromatin which maintains transcriptional repression established during early development in many eukaryotes. However, the mechanism underlying establishment and regulation of epigenetic asymmetry in the zygote remains obscure. Here we show that maternal EZH2 is required for the establishment of H3K27me3 in mouse zygotes. However, combined immunostaining with ULI-NChIP-seq (ultra-low-input micrococcal nuclease-based native ChIP-seq) shows that EZH1 could partially safeguard the role of EZH2 in the formation of H3K27me2. Meanwhile, we identify that EHMT1 is involved in the establishment of H3K27me2, and that H3K27me2 might be an essential prerequisite for the following de novo H3K27me3 modification on the male pronucleus. In this work, we clarify the establishment and regulatory mechanisms of H3K27me2 and H3K27me3 in mouse zygotes.

Suggested Citation

  • Tie-Gang Meng & Qian Zhou & Xue-Shan Ma & Xiao-Yu Liu & Qing-Ren Meng & Xian-Ju Huang & Hong-Lin Liu & Wen-Long Lei & Zheng-Hui Zhao & Ying-Chun Ouyang & Yi Hou & Heide Schatten & Xiang-Hong Ou & Zhen, 2020. "PRC2 and EHMT1 regulate H3K27me2 and H3K27me3 establishment across the zygote genome," Nature Communications, Nature, vol. 11(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-20242-9
    DOI: 10.1038/s41467-020-20242-9
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    Cited by:

    1. Jiang Zhu & Kang Chen & Yu H. Sun & Wen Ye & Juntao Liu & Dandan Zhang & Nan Su & Li Wu & Xiaochen Kou & Yanhong Zhao & Hong Wang & Shaorong Gao & Lan Kang, 2023. "LSM1-mediated Major Satellite RNA decay is required for nonequilibrium histone H3.3 incorporation into parental pronuclei," Nature Communications, Nature, vol. 14(1), pages 1-16, December.

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