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TBPL2/TFIIA complex establishes the maternal transcriptome through oocyte-specific promoter usage

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  • Changwei Yu

    (Institut de Génétique et de Biologie Moléculaire et Cellulaire
    Centre National de la Recherche Scientifique (CNRS), UMR7104
    Institut National de la Santé et de la Recherche Médicale (INSERM), U1258
    Université de Strasbourg)

  • Nevena Cvetesic

    (Imperial College London, South Kensington Campus)

  • Vincent Hisler

    (Institut de Génétique et de Biologie Moléculaire et Cellulaire
    Centre National de la Recherche Scientifique (CNRS), UMR7104
    Institut National de la Santé et de la Recherche Médicale (INSERM), U1258
    Université de Strasbourg)

  • Kapil Gupta

    (University of Bristol, Cantock’s Close)

  • Tao Ye

    (Institut de Génétique et de Biologie Moléculaire et Cellulaire
    Centre National de la Recherche Scientifique (CNRS), UMR7104
    Institut National de la Santé et de la Recherche Médicale (INSERM), U1258
    Université de Strasbourg)

  • Emese Gazdag

    (Institut de Génétique et de Biologie Moléculaire et Cellulaire
    Centre National de la Recherche Scientifique (CNRS), UMR7104
    Institut National de la Santé et de la Recherche Médicale (INSERM), U1258
    Université de Strasbourg)

  • Luc Negroni

    (Institut de Génétique et de Biologie Moléculaire et Cellulaire
    Centre National de la Recherche Scientifique (CNRS), UMR7104
    Institut National de la Santé et de la Recherche Médicale (INSERM), U1258
    Université de Strasbourg)

  • Petra Hajkova

    (Imperial College London, South Kensington Campus)

  • Imre Berger

    (University of Bristol, Cantock’s Close)

  • Boris Lenhard

    (Imperial College London, South Kensington Campus)

  • Ferenc Müller

    (University of Birmingham)

  • Stéphane D. Vincent

    (Institut de Génétique et de Biologie Moléculaire et Cellulaire
    Centre National de la Recherche Scientifique (CNRS), UMR7104
    Institut National de la Santé et de la Recherche Médicale (INSERM), U1258
    Université de Strasbourg)

  • László Tora

    (Institut de Génétique et de Biologie Moléculaire et Cellulaire
    Centre National de la Recherche Scientifique (CNRS), UMR7104
    Institut National de la Santé et de la Recherche Médicale (INSERM), U1258
    Université de Strasbourg)

Abstract

During oocyte growth, transcription is required to create RNA and protein reserves to achieve maternal competence. During this period, the general transcription factor TATA binding protein (TBP) is replaced by its paralogue, TBPL2 (TBP2 or TRF3), which is essential for RNA polymerase II transcription. We show that in oocytes TBPL2 does not assemble into a canonical TFIID complex. Our transcript analyses demonstrate that TBPL2 mediates transcription of oocyte-expressed genes, including mRNA survey genes, as well as specific endogenous retroviral elements. Transcription start site (TSS) mapping indicates that TBPL2 has a strong preference for TATA-like motif in core promoters driving sharp TSS selection, in contrast with canonical TBP/TFIID-driven TATA-less promoters that have broader TSS architecture. Thus, we show a role for the TBPL2/TFIIA complex in the establishment of the oocyte transcriptome by using a specific TSS recognition code.

Suggested Citation

  • Changwei Yu & Nevena Cvetesic & Vincent Hisler & Kapil Gupta & Tao Ye & Emese Gazdag & Luc Negroni & Petra Hajkova & Imre Berger & Boris Lenhard & Ferenc Müller & Stéphane D. Vincent & László Tora, 2020. "TBPL2/TFIIA complex establishes the maternal transcriptome through oocyte-specific promoter usage," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-20239-4
    DOI: 10.1038/s41467-020-20239-4
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