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Bis(zinc(II)-dipicolylamine)-functionalized sub-2 μm core-shell microspheres for the analysis of N-phosphoproteome

Author

Listed:
  • Yechen Hu

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences
    Nanjing Medical University)

  • Bo Jiang

    (Chinese Academy of Sciences)

  • Yejing Weng

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Zhigang Sui

    (Chinese Academy of Sciences)

  • Baofeng Zhao

    (Chinese Academy of Sciences)

  • Yuanbo Chen

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Lukuan Liu

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Qiong Wu

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Zhen Liang

    (Chinese Academy of Sciences)

  • Lihua Zhang

    (Chinese Academy of Sciences)

  • Yukui Zhang

    (Chinese Academy of Sciences)

Abstract

Protein N-phosphorylation plays a critical role in central metabolism and two/multicomponent signaling of prokaryotes. However, the current enrichment methods for O-phosphopeptides are not preferred for N-phosphopeptides due to the intrinsic lability of P-N bond under acidic conditions. Therefore, the effective N-phosphoproteome analysis remains challenging. Herein, bis(zinc(II)-dipicolylamine)-functionalized sub-2 μm core-shell silica microspheres (SiO2@DpaZn) are tailored for rapid and effective N-phosphopeptides enrichment. Due to the coordination of phosphate groups to Zn(II), N-phosphopeptides can be effectively captured under neutral conditions. Moreover, the method is successfully applied to an E.coli and HeLa N-phosphoproteome study. These results further broaden the range of methods for the discovery of N-phosphoproteins with significant biological functions.

Suggested Citation

  • Yechen Hu & Bo Jiang & Yejing Weng & Zhigang Sui & Baofeng Zhao & Yuanbo Chen & Lukuan Liu & Qiong Wu & Zhen Liang & Lihua Zhang & Yukui Zhang, 2020. "Bis(zinc(II)-dipicolylamine)-functionalized sub-2 μm core-shell microspheres for the analysis of N-phosphoproteome," Nature Communications, Nature, vol. 11(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-20026-1
    DOI: 10.1038/s41467-020-20026-1
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