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An expanded palette of improved SPLICS reporters detects multiple organelle contacts in vitro and in vivo

Author

Listed:
  • Francesca Vallese

    (University of Padova)

  • Cristina Catoni

    (University of Padova)

  • Domenico Cieri

    (University of Padova)

  • Lucia Barazzuol

    (University of Padova)

  • Omar Ramirez

    (Interdisciplinary Center for Neurosciences, Heidelberg University)

  • Valentina Calore

    (University of Padova)

  • Massimo Bonora

    (Surgery and Experimental Medicine, Section of General Pathology, University of Ferrara
    Laboratory for Technologies of Advanced Therapies (LTTA), University of Ferrara)

  • Flavia Giamogante

    (University of Padova)

  • Paolo Pinton

    (Surgery and Experimental Medicine, Section of General Pathology, University of Ferrara
    Laboratory for Technologies of Advanced Therapies (LTTA), University of Ferrara)

  • Marisa Brini

    (University of Padova)

  • Tito Calì

    (University of Padova
    Padova Neuroscience Center (PNC), University of Padova)

Abstract

Membrane contact sites between virtually any known organelle have been documented and, in the last decades, their study received momentum due to their importance for fundamental activities of the cell and for the subtle comprehension of many human diseases. The lack of tools to finely image inter-organelle proximity hindered our understanding on how these subcellular communication hubs mediate and regulate cell homeostasis. We develop an improved and expanded palette of split-GFP-based contact site sensors (SPLICS) for the detection of single and multiple organelle contact sites within a scalable distance range. We demonstrate their flexibility under physiological conditions and in living organisms.

Suggested Citation

  • Francesca Vallese & Cristina Catoni & Domenico Cieri & Lucia Barazzuol & Omar Ramirez & Valentina Calore & Massimo Bonora & Flavia Giamogante & Paolo Pinton & Marisa Brini & Tito Calì, 2020. "An expanded palette of improved SPLICS reporters detects multiple organelle contacts in vitro and in vivo," Nature Communications, Nature, vol. 11(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-19892-6
    DOI: 10.1038/s41467-020-19892-6
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    Cited by:

    1. Katelyn C. Cook & Elene Tsopurashvili & Jason M. Needham & Sunnie R. Thompson & Ileana M. Cristea, 2022. "Restructured membrane contacts rewire organelles for human cytomegalovirus infection," Nature Communications, Nature, vol. 13(1), pages 1-20, December.
    2. Flavia Giamogante & Lucia Barazzuol & Francesca Maiorca & Elena Poggio & Alessandra Esposito & Anna Masato & Gennaro Napolitano & Alessio Vagnoni & Tito Calì & Marisa Brini, 2024. "A SPLICS reporter reveals $${{{{{\boldsymbol{\alpha }}}}}}$$ α -synuclein regulation of lysosome-mitochondria contacts which affects TFEB nuclear translocation," Nature Communications, Nature, vol. 15(1), pages 1-20, December.
    3. Maria Casas & Karl D. Murray & Keiko Hino & Nicholas C. Vierra & Sergi Simó & James S. Trimmer & Rose E. Dixon & Eamonn J. Dickson, 2023. "NPC1-dependent alterations in KV2.1–CaV1.2 nanodomains drive neuronal death in models of Niemann-Pick Type C disease," Nature Communications, Nature, vol. 14(1), pages 1-22, December.
    4. Eunbyul Cho & Youngsik Woo & Yeongjun Suh & Bo Kyoung Suh & Soo Jeong Kim & Truong Thi My Nhung & Jin Yeong Yoo & Tran Diem Nghi & Su Been Lee & Dong Jin Mun & Sang Ki Park, 2023. "Ratiometric measurement of MAM Ca2+ dynamics using a modified CalfluxVTN," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

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