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RNA-mediated control of cell shape modulates antibiotic resistance in Vibrio cholerae

Author

Listed:
  • Nikolai Peschek

    (Friedrich Schiller University
    Ludwig-Maximilians-University of Munich)

  • Roman Herzog

    (Friedrich Schiller University
    Ludwig-Maximilians-University of Munich)

  • Praveen K. Singh

    (Max Planck Institute for Terrestrial Microbiology)

  • Marcel Sprenger

    (Friedrich Schiller University)

  • Fabian Meyer

    (Ludwig-Maximilians-University of Munich
    Christian-Albrechts-University)

  • Kathrin S. Fröhlich

    (Friedrich Schiller University
    Ludwig-Maximilians-University of Munich
    Friedrich Schiller University Jena)

  • Luise Schröger

    (Ludwig-Maximilians-University of Munich)

  • Marc Bramkamp

    (Ludwig-Maximilians-University of Munich
    Christian-Albrechts-University)

  • Knut Drescher

    (Max Planck Institute for Terrestrial Microbiology
    Philipps-Universität Marburg)

  • Kai Papenfort

    (Friedrich Schiller University
    Ludwig-Maximilians-University of Munich
    Friedrich Schiller University Jena)

Abstract

Vibrio cholerae, the cause of cholera disease, exhibits a characteristic curved rod morphology, which promotes infectivity and motility in dense hydrogels. Periplasmic protein CrvA determines cell curvature in V. cholerae, yet the regulatory factors controlling CrvA are unknown. Here, we discover the VadR small RNA (sRNA) as a post-transcriptional inhibitor of the crvA mRNA. Mutation of vadR increases cell curvature, whereas overexpression has the inverse effect. We show that vadR transcription is activated by the VxrAB two-component system and triggered by cell-wall-targeting antibiotics. V. cholerae cells failing to repress crvA by VadR display decreased survival upon challenge with penicillin G indicating that cell shape maintenance by the sRNA is critical for antibiotic resistance. VadR also blocks the expression of various key biofilm genes and thereby inhibits biofilm formation in V. cholerae. Thus, VadR is an important regulator for synchronizing peptidoglycan integrity, cell shape, and biofilm formation in V. cholerae.

Suggested Citation

  • Nikolai Peschek & Roman Herzog & Praveen K. Singh & Marcel Sprenger & Fabian Meyer & Kathrin S. Fröhlich & Luise Schröger & Marc Bramkamp & Knut Drescher & Kai Papenfort, 2020. "RNA-mediated control of cell shape modulates antibiotic resistance in Vibrio cholerae," Nature Communications, Nature, vol. 11(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-19890-8
    DOI: 10.1038/s41467-020-19890-8
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    Cited by:

    1. Michaela Huber & Anne Lippegaus & Sahar Melamed & Malte Siemers & Benjamin R. Wucher & Mona Hoyos & Carey Nadell & Gisela Storz & Kai Papenfort, 2022. "An RNA sponge controls quorum sensing dynamics and biofilm formation in Vibrio cholerae," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    2. Fang Liu & Ziying Chen & Shuo Zhang & Kejing Wu & Cheng Bei & Chuan Wang & Yanjie Chao, 2023. "In vivo RNA interactome profiling reveals 3’UTR-processed small RNA targeting a central regulatory hub," Nature Communications, Nature, vol. 14(1), pages 1-15, December.

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