IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v11y2020i1d10.1038_s41467-020-19890-8.html
   My bibliography  Save this article

RNA-mediated control of cell shape modulates antibiotic resistance in Vibrio cholerae

Author

Listed:
  • Nikolai Peschek

    (Friedrich Schiller University
    Ludwig-Maximilians-University of Munich)

  • Roman Herzog

    (Friedrich Schiller University
    Ludwig-Maximilians-University of Munich)

  • Praveen K. Singh

    (Max Planck Institute for Terrestrial Microbiology)

  • Marcel Sprenger

    (Friedrich Schiller University)

  • Fabian Meyer

    (Ludwig-Maximilians-University of Munich
    Christian-Albrechts-University)

  • Kathrin S. Fröhlich

    (Friedrich Schiller University
    Ludwig-Maximilians-University of Munich
    Friedrich Schiller University Jena)

  • Luise Schröger

    (Ludwig-Maximilians-University of Munich)

  • Marc Bramkamp

    (Ludwig-Maximilians-University of Munich
    Christian-Albrechts-University)

  • Knut Drescher

    (Max Planck Institute for Terrestrial Microbiology
    Philipps-Universität Marburg)

  • Kai Papenfort

    (Friedrich Schiller University
    Ludwig-Maximilians-University of Munich
    Friedrich Schiller University Jena)

Abstract

Vibrio cholerae, the cause of cholera disease, exhibits a characteristic curved rod morphology, which promotes infectivity and motility in dense hydrogels. Periplasmic protein CrvA determines cell curvature in V. cholerae, yet the regulatory factors controlling CrvA are unknown. Here, we discover the VadR small RNA (sRNA) as a post-transcriptional inhibitor of the crvA mRNA. Mutation of vadR increases cell curvature, whereas overexpression has the inverse effect. We show that vadR transcription is activated by the VxrAB two-component system and triggered by cell-wall-targeting antibiotics. V. cholerae cells failing to repress crvA by VadR display decreased survival upon challenge with penicillin G indicating that cell shape maintenance by the sRNA is critical for antibiotic resistance. VadR also blocks the expression of various key biofilm genes and thereby inhibits biofilm formation in V. cholerae. Thus, VadR is an important regulator for synchronizing peptidoglycan integrity, cell shape, and biofilm formation in V. cholerae.

Suggested Citation

  • Nikolai Peschek & Roman Herzog & Praveen K. Singh & Marcel Sprenger & Fabian Meyer & Kathrin S. Fröhlich & Luise Schröger & Marc Bramkamp & Knut Drescher & Kai Papenfort, 2020. "RNA-mediated control of cell shape modulates antibiotic resistance in Vibrio cholerae," Nature Communications, Nature, vol. 11(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-19890-8
    DOI: 10.1038/s41467-020-19890-8
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-020-19890-8
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-020-19890-8?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Michaela Huber & Anne Lippegaus & Sahar Melamed & Malte Siemers & Benjamin R. Wucher & Mona Hoyos & Carey Nadell & Gisela Storz & Kai Papenfort, 2022. "An RNA sponge controls quorum sensing dynamics and biofilm formation in Vibrio cholerae," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    2. Fang Liu & Ziying Chen & Shuo Zhang & Kejing Wu & Cheng Bei & Chuan Wang & Yanjie Chao, 2023. "In vivo RNA interactome profiling reveals 3’UTR-processed small RNA targeting a central regulatory hub," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    3. Sebastian Pöhl & Giacomo Giacomelli & Fabian M. Meyer & Volker Kleeberg & Eli J. Cohen & Jacob Biboy & Julia Rosum & Timo Glatter & Waldemar Vollmer & Muriel C. F. Teeseling & Johann Heider & Marc Bra, 2024. "An outer membrane porin-lipoprotein complex modulates elongasome movement to establish cell curvature in Rhodospirillum rubrum," Nature Communications, Nature, vol. 15(1), pages 1-21, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-19890-8. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.