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In vivo biomolecular imaging of zebrafish embryos using confocal Raman spectroscopy

Author

Listed:
  • Håkon Høgset

    (Imperial College London)

  • Conor C. Horgan

    (Imperial College London)

  • James P. K. Armstrong

    (Imperial College London)

  • Mads S. Bergholt

    (Imperial College London
    Kings College London, Tower Wing, Guy’s Hospital)

  • Vincenzo Torraca

    (London School of Hygiene & Tropical Medicine)

  • Qu Chen

    (Imperial College London)

  • Timothy J. Keane

    (Imperial College London)

  • Laurence Bugeon

    (Imperial College London)

  • Margaret J. Dallman

    (Imperial College London)

  • Serge Mostowy

    (London School of Hygiene & Tropical Medicine)

  • Molly M. Stevens

    (Imperial College London)

Abstract

Zebrafish embryos provide a unique opportunity to visualize complex biological processes, yet conventional imaging modalities are unable to access intricate biomolecular information without compromising the integrity of the embryos. Here, we report the use of confocal Raman spectroscopic imaging for the visualization and multivariate analysis of biomolecular information extracted from unlabeled zebrafish embryos. We outline broad applications of this method in: (i) visualizing the biomolecular distribution of whole embryos in three dimensions, (ii) resolving anatomical features at subcellular spatial resolution, (iii) biomolecular profiling and discrimination of wild type and ΔRD1 mutant Mycobacterium marinum strains in a zebrafish embryo model of tuberculosis and (iv) in vivo temporal monitoring of the wound response in living zebrafish embryos. Overall, this study demonstrates the application of confocal Raman spectroscopic imaging for the comparative bimolecular analysis of fully intact and living zebrafish embryos.

Suggested Citation

  • Håkon Høgset & Conor C. Horgan & James P. K. Armstrong & Mads S. Bergholt & Vincenzo Torraca & Qu Chen & Timothy J. Keane & Laurence Bugeon & Margaret J. Dallman & Serge Mostowy & Molly M. Stevens, 2020. "In vivo biomolecular imaging of zebrafish embryos using confocal Raman spectroscopy," Nature Communications, Nature, vol. 11(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-19827-1
    DOI: 10.1038/s41467-020-19827-1
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    Cited by:

    1. Hao He & Maofeng Cao & Yun Gao & Peng Zheng & Sen Yan & Jin-Hui Zhong & Lei Wang & Dayong Jin & Bin Ren, 2024. "Noise learning of instruments for high-contrast, high-resolution and fast hyperspectral microscopy and nanoscopy," Nature Communications, Nature, vol. 15(1), pages 1-12, December.

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