IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v11y2020i1d10.1038_s41467-020-19787-6.html
   My bibliography  Save this article

Direct RNA sequencing reveals m6A modifications on adenovirus RNA are necessary for efficient splicing

Author

Listed:
  • Alexander M. Price

    (The Children’s Hospital of Philadelphia)

  • Katharina E. Hayer

    (The Children’s Hospital of Philadelphia)

  • Alexa B. R. McIntyre

    (Weill Cornell Medicine
    Tri-Institutional Program in Computational Biology and Medicine
    University of Zurich)

  • Nandan S. Gokhale

    (Duke University Medical Center
    University of Washington)

  • Jonathan S. Abebe

    (New York University School of Medicine)

  • Ashley N. Fera

    (The Children’s Hospital of Philadelphia
    University of Maryland)

  • Christopher E. Mason

    (Weill Cornell Medicine
    Weill Cornell Medicine
    Weill Cornell Medicine
    Weill Cornell Medicine)

  • Stacy M. Horner

    (Duke University Medical Center
    Duke University Medical Center)

  • Angus C. Wilson

    (New York University School of Medicine)

  • Daniel P. Depledge

    (New York University School of Medicine)

  • Matthew D. Weitzman

    (The Children’s Hospital of Philadelphia
    University of Pennsylvania Perelman School of Medicine)

Abstract

Adenovirus is a nuclear replicating DNA virus reliant on host RNA processing machinery. Processing and metabolism of cellular RNAs can be regulated by METTL3, which catalyzes the addition of N6-methyladenosine (m6A) to mRNAs. While m6A-modified adenoviral RNAs have been previously detected, the location and function of this mark within the infectious cycle is unknown. Since the complex adenovirus transcriptome includes overlapping spliced units that would impede accurate m6A mapping using short-read sequencing, here we profile m6A within the adenovirus transcriptome using a combination of meRIP-seq and direct RNA long-read sequencing to yield both nucleotide and transcript-resolved m6A detection. Although both early and late viral transcripts contain m6A, depletion of m6A writer METTL3 specifically impacts viral late transcripts by reducing their splicing efficiency. These data showcase a new technique for m6A discovery within individual transcripts at nucleotide resolution, and highlight the role of m6A in regulating splicing of a viral pathogen.

Suggested Citation

  • Alexander M. Price & Katharina E. Hayer & Alexa B. R. McIntyre & Nandan S. Gokhale & Jonathan S. Abebe & Ashley N. Fera & Christopher E. Mason & Stacy M. Horner & Angus C. Wilson & Daniel P. Depledge , 2020. "Direct RNA sequencing reveals m6A modifications on adenovirus RNA are necessary for efficient splicing," Nature Communications, Nature, vol. 11(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-19787-6
    DOI: 10.1038/s41467-020-19787-6
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-020-19787-6
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-020-19787-6?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. P Acera Mateos & A J Sethi & A Ravindran & A Srivastava & K Woodward & S Mahmud & M Kanchi & M Guarnacci & J Xu & Z W S Yuen & Y Zhou & A Sneddon & W Hamilton & J Gao & L M Starrs & R Hayashi & V Wick, 2024. "Prediction of m6A and m5C at single-molecule resolution reveals a transcriptome-wide co-occurrence of RNA modifications," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    2. Adrien Leger & Paulo P. Amaral & Luca Pandolfini & Charlotte Capitanchik & Federica Capraro & Valentina Miano & Valentina Migliori & Patrick Toolan-Kerr & Theodora Sideri & Anton J. Enright & Konstant, 2021. "RNA modifications detection by comparative Nanopore direct RNA sequencing," Nature Communications, Nature, vol. 12(1), pages 1-17, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-19787-6. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.