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High-dimensional super-resolution imaging reveals heterogeneity and dynamics of subcellular lipid membranes

Author

Listed:
  • Karl Zhanghao

    (Southern University of Science and Technology
    Peking University)

  • Wenhui Liu

    (Tsinghua University)

  • Meiqi Li

    (Peking University)

  • Zihan Wu

    (Southern University of Science and Technology)

  • Xiao Wang

    (Peking University)

  • Xingye Chen

    (Tsinghua University)

  • Chunyan Shan

    (Peking University)

  • Haoqian Wang

    (Tsinghua University)

  • Xiaowei Chen

    (Peking University)

  • Qionghai Dai

    (Tsinghua University)

  • Peng Xi

    (Southern University of Science and Technology
    Peking University)

  • Dayong Jin

    (Southern University of Science and Technology
    University of Technology Sydney)

Abstract

Lipid membranes are found in most intracellular organelles, and their heterogeneities play an essential role in regulating the organelles’ biochemical functionalities. Here we report a Spectrum and Polarization Optical Tomography (SPOT) technique to study the subcellular lipidomics in live cells. Simply using one dye that universally stains the lipid membranes, SPOT can simultaneously resolve the membrane morphology, polarity, and phase from the three optical-dimensions of intensity, spectrum, and polarization, respectively. These high-throughput optical properties reveal lipid heterogeneities of ten subcellular compartments, at different developmental stages, and even within the same organelle. Furthermore, we obtain real-time monitoring of the multi-organelle interactive activities of cell division and successfully reveal their sophisticated lipid dynamics during the plasma membrane separation, tunneling nanotubules formation, and mitochondrial cristae dissociation. This work suggests research frontiers in correlating single-cell super-resolution lipidomics with multiplexed imaging of organelle interactome.

Suggested Citation

  • Karl Zhanghao & Wenhui Liu & Meiqi Li & Zihan Wu & Xiao Wang & Xingye Chen & Chunyan Shan & Haoqian Wang & Xiaowei Chen & Qionghai Dai & Peng Xi & Dayong Jin, 2020. "High-dimensional super-resolution imaging reveals heterogeneity and dynamics of subcellular lipid membranes," Nature Communications, Nature, vol. 11(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-19747-0
    DOI: 10.1038/s41467-020-19747-0
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    Cited by:

    1. Marko D. Petrović & Ilija Milovanović & Tamara Gajić & Veronika N. Kholina & Miroslav Vujičić & Ivana Blešić & Filip Đoković & Milan M. Radovanović & Nina B. Ćurčić & Al Fauzi Rahmat & Karlygash Muzdy, 2023. "The Degree of Environmental Risk and Attractiveness as a Criterion for Visiting a Tourist Destination," Sustainability, MDPI, vol. 15(19), pages 1-20, September.

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