IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v11y2020i1d10.1038_s41467-020-19650-8.html
   My bibliography  Save this article

Vaccine elicitation of HIV broadly neutralizing antibodies from engineered B cells

Author

Listed:
  • Deli Huang

    (The Scripps Research Institute)

  • Jenny Tuyet Tran

    (The Scripps Research Institute)

  • Alex Olson

    (The Scripps Research Institute)

  • Thomas Vollbrecht

    (The University of California San Diego)

  • Mary Tenuta

    (The Scripps Research Institute)

  • Mariia V. Guryleva

    (Tumor and Cell Biology, Karolinska Institutet
    Moscow Lomonosov State University)

  • Roberta P. Fuller

    (The Scripps Research Institute
    The Scripps Research Institute
    The Scripps Research Institute)

  • Torben Schiffner

    (The Scripps Research Institute
    The Scripps Research Institute
    The Scripps Research Institute)

  • Justin R. Abadejos

    (The Scripps Research Institute)

  • Lauren Couvrette

    (The Scripps Research Institute
    University of Ottawa)

  • Tanya R. Blane

    (The Scripps Research Institute)

  • Karen Saye

    (The Scripps Research Institute
    The Scripps Research Institute
    The Scripps Research Institute)

  • Wenjuan Li

    (The Scripps Research Institute)

  • Elise Landais

    (The Scripps Research Institute
    The Scripps Research Institute)

  • Alicia Gonzalez-Martin

    (Universidad Autónoma de Madrid (UAM) and Instituto de Investigaciones Biomédicas Alberto Sols (CSIC-UAM))

  • William Schief

    (The Scripps Research Institute
    The Scripps Research Institute
    The Scripps Research Institute
    Massachusetts Institute of Technology, and Harvard)

  • Ben Murrell

    (Tumor and Cell Biology, Karolinska Institutet)

  • Dennis R. Burton

    (The Scripps Research Institute
    The Scripps Research Institute
    The Scripps Research Institute
    Massachusetts Institute of Technology, and Harvard)

  • David Nemazee

    (The Scripps Research Institute)

  • James E. Voss

    (The Scripps Research Institute)

Abstract

HIV broadly neutralizing antibodies (bnAbs) can suppress viremia and protect against HIV infection. However, their elicitation is made difficult by low frequencies of appropriate precursor B cell receptors and the complex maturation pathways required to generate bnAbs from these precursors. Antibody genes can be engineered into B cells for expression as both a functional antigen receptor on cell surfaces and as secreted antibody. Here, we show that HIV bnAb-engineered primary mouse B cells can be adoptively transferred and vaccinated in immunocompetent mice resulting in the expansion of durable bnAb memory and long-lived plasma cells. Somatic hypermutation after immunization indicates that engineered cells have the capacity to respond to an evolving pathogen. These results encourage further exploration of engineered B cell vaccines as a strategy for durable elicitation of HIV bnAbs to protect against infection and as a contributor to a functional HIV cure.

Suggested Citation

  • Deli Huang & Jenny Tuyet Tran & Alex Olson & Thomas Vollbrecht & Mary Tenuta & Mariia V. Guryleva & Roberta P. Fuller & Torben Schiffner & Justin R. Abadejos & Lauren Couvrette & Tanya R. Blane & Kare, 2020. "Vaccine elicitation of HIV broadly neutralizing antibodies from engineered B cells," Nature Communications, Nature, vol. 11(1), pages 1-10, December.
  • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-19650-8
    DOI: 10.1038/s41467-020-19650-8
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-020-19650-8
    File Function: Abstract
    Download Restriction: no

    File URL: https://libkey.io/10.1038/s41467-020-19650-8?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Rene Yu-Hong Cheng & King L. Hung & Tingting Zhang & Claire M. Stoffers & Andee R. Ott & Emmaline R. Suchland & Nathan D. Camp & Iram F. Khan & Swati Singh & Ying-Jen Yang & David J. Rawlings & Richar, 2022. "Ex vivo engineered human plasma cells exhibit robust protein secretion and long-term engraftment in vivo," Nature Communications, Nature, vol. 13(1), pages 1-14, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-19650-8. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.