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Subnuclear gene positioning through lamina association affects copper tolerance

Author

Listed:
  • Yuki Sakamoto

    (Tokyo University of Science
    Osaka University)

  • Mayuko Sato

    (RIKEN Center for Sustainable Resource Science)

  • Yoshikatsu Sato

    (Nagoya University)

  • Akihito Harada

    (Kyushu University)

  • Takamasa Suzuki

    (Chubu University)

  • Chieko Goto

    (Graduate School of Agricultural and Life Sciences, The University of Tokyo
    Kobe University)

  • Kentaro Tamura

    (University of Shizuoka)

  • Kiminori Toyooka

    (RIKEN Center for Sustainable Resource Science)

  • Hiroshi Kimura

    (Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology)

  • Yasuyuki Ohkawa

    (Kyushu University)

  • Ikuko Hara-Nishimura

    (Konan University)

  • Shingo Takagi

    (Osaka University)

  • Sachihiro Matsunaga

    (Tokyo University of Science
    Tokyo University of Science
    The University of Tokyo)

Abstract

The nuclear lamina plays an important role in the regulation of chromatin organization and gene positioning in animals. CROWDED NUCLEI (CRWN) is a strong candidate for the plant nuclear lamina protein in Arabidopsis thaliana but its biological function was largely unknown. Here, we show that CRWNs localize at the nuclear lamina and build the meshwork structure. Fluorescence in situ hybridization and RNA-seq analyses revealed that CRWNs regulate chromatin distribution and gene expression. More than 2000 differentially expressed genes were identified in the crwn1crwn4 double mutant. Copper-associated (CA) genes that form a gene cluster on chromosome 5 were among the downregulated genes in the double mutant exhibiting low tolerance to excess copper. Our analyses showed this low tolerance to copper was associated with the suppression of CA gene expression and that CRWN1 interacts with the CA gene locus, enabling the locus to localize at the nuclear lamina under excess copper conditions.

Suggested Citation

  • Yuki Sakamoto & Mayuko Sato & Yoshikatsu Sato & Akihito Harada & Takamasa Suzuki & Chieko Goto & Kentaro Tamura & Kiminori Toyooka & Hiroshi Kimura & Yasuyuki Ohkawa & Ikuko Hara-Nishimura & Shingo Ta, 2020. "Subnuclear gene positioning through lamina association affects copper tolerance," Nature Communications, Nature, vol. 11(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-19621-z
    DOI: 10.1038/s41467-020-19621-z
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    Cited by:

    1. Laurence Cromer & Mariana Tiscareno-Andrade & Sandrine Lefranc & Aurélie Chambon & Aurélie Hurel & Manon Brogniez & Julie Guérin & Ivan Masson & Gabriele Adam & Delphine Charif & Philippe Andrey & Mat, 2024. "Rapid meiotic prophase chromosome movements in Arabidopsis thaliana are linked to essential reorganization at the nuclear envelope," Nature Communications, Nature, vol. 15(1), pages 1-21, December.

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