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Organized cannabinoid receptor distribution in neurons revealed by super-resolution fluorescence imaging

Author

Listed:
  • Hui Li

    (ShanghaiTech University)

  • Jie Yang

    (ShanghaiTech University
    University of the Chinese Academy of Sciences
    ShanghaiTech University)

  • Cuiping Tian

    (ShanghaiTech University)

  • Min Diao

    (ShanghaiTech University)

  • Quan Wang

    (ShanghaiTech University
    University of the Chinese Academy of Sciences
    ShanghaiTech University)

  • Simeng Zhao

    (ShanghaiTech University)

  • Shanshan Li

    (ShanghaiTech University)

  • Fangzhi Tan

    (ShanghaiTech University)

  • Tian Hua

    (ShanghaiTech University)

  • Ya Qin

    (ShanghaiTech University)

  • Chao-Po Lin

    (ShanghaiTech University)

  • Dylan Deska-Gauthier

    (Dalhousie University)

  • Garth J. Thompson

    (ShanghaiTech University)

  • Ying Zhang

    (Dalhousie University)

  • Wenqing Shui

    (ShanghaiTech University
    ShanghaiTech University)

  • Zhi-Jie Liu

    (ShanghaiTech University
    ShanghaiTech University)

  • Tong Wang

    (ShanghaiTech University)

  • Guisheng Zhong

    (ShanghaiTech University
    ShanghaiTech University)

Abstract

G-protein-coupled receptors (GPCRs) play important roles in cellular functions. However, their intracellular organization is largely unknown. Through investigation of the cannabinoid receptor 1 (CB1), we discovered periodically repeating clusters of CB1 hotspots within the axons of neurons. We observed these CB1 hotspots interact with the membrane-associated periodic skeleton (MPS) forming a complex crucial in the regulation of CB1 signaling. Furthermore, we found that CB1 hotspot periodicity increased upon CB1 agonist application, and these activated CB1 displayed less dynamic movement compared to non-activated CB1. Our results suggest that CB1 forms periodic hotspots organized by the MPS as a mechanism to increase signaling efficacy upon activation.

Suggested Citation

  • Hui Li & Jie Yang & Cuiping Tian & Min Diao & Quan Wang & Simeng Zhao & Shanshan Li & Fangzhi Tan & Tian Hua & Ya Qin & Chao-Po Lin & Dylan Deska-Gauthier & Garth J. Thompson & Ying Zhang & Wenqing Sh, 2020. "Organized cannabinoid receptor distribution in neurons revealed by super-resolution fluorescence imaging," Nature Communications, Nature, vol. 11(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-19510-5
    DOI: 10.1038/s41467-020-19510-5
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