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The smfBox is an open-source platform for single-molecule FRET

Author

Listed:
  • Benjamin Ambrose

    (University of Sheffield)

  • James M. Baxter

    (University of Sheffield)

  • John Cully

    (University of Sheffield)

  • Matthew Willmott

    (University of Sheffield)

  • Elliot M. Steele

    (University of Sheffield)

  • Benji C. Bateman

    (Central Laser Facility, Research Complex at Harwell, Rutherford Appleton Laboratory)

  • Marisa L. Martin-Fernandez

    (Central Laser Facility, Research Complex at Harwell, Rutherford Appleton Laboratory)

  • Ashley Cadby

    (University of Sheffield)

  • Jonathan Shewring

    (University of Sheffield)

  • Marleen Aaldering

    (University of Sheffield)

  • Timothy D. Craggs

    (University of Sheffield)

Abstract

Single-molecule Förster Resonance Energy Transfer (smFRET) is a powerful technique capable of resolving both relative and absolute distances within and between structurally dynamic biomolecules. High instrument costs, and a lack of open-source hardware and acquisition software have limited smFRET’s broad application by non-specialists. Here, we present the smfBox, a cost-effective confocal smFRET platform, providing detailed build instructions, open-source acquisition software, and full validation, thereby democratising smFRET for the wider scientific community.

Suggested Citation

  • Benjamin Ambrose & James M. Baxter & John Cully & Matthew Willmott & Elliot M. Steele & Benji C. Bateman & Marisa L. Martin-Fernandez & Ashley Cadby & Jonathan Shewring & Marleen Aaldering & Timothy D, 2020. "The smfBox is an open-source platform for single-molecule FRET," Nature Communications, Nature, vol. 11(1), pages 1-6, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-19468-4
    DOI: 10.1038/s41467-020-19468-4
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    Cited by:

    1. Andreas Hartmann & Koushik Sreenivasa & Mathias Schenkel & Neharika Chamachi & Philipp Schake & Georg Krainer & Michael Schlierf, 2023. "An automated single-molecule FRET platform for high-content, multiwell plate screening of biomolecular conformations and dynamics," Nature Communications, Nature, vol. 14(1), pages 1-12, December.

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