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A versatile and customizable low-cost 3D-printed open standard for microscopic imaging

Author

Listed:
  • Benedict Diederich

    (Leibniz Institute of Photonic Technology
    Institute of Physical Chemistry and Abbe Center of Photonics, Helmholtzweg 4, Friedrich-Schiller-University)

  • René Lachmann

    (Leibniz Institute of Photonic Technology
    Faculty of Physics and Astronomy, Friedrich-Schiller-University)

  • Swen Carlstedt

    (Jena University Hospital, Institute of Biochemistry II)

  • Barbora Marsikova

    (Leibniz Institute of Photonic Technology
    Faculty of Physics and Astronomy, Friedrich-Schiller-University)

  • Haoran Wang

    (Leibniz Institute of Photonic Technology)

  • Xavier Uwurukundo

    (Leibniz Institute of Photonic Technology)

  • Alexander S. Mosig

    (Jena University Hospital, Institute of Biochemistry II)

  • Rainer Heintzmann

    (Leibniz Institute of Photonic Technology
    Institute of Physical Chemistry and Abbe Center of Photonics, Helmholtzweg 4, Friedrich-Schiller-University
    Faculty of Physics and Astronomy, Friedrich-Schiller-University)

Abstract

Modern microscopes used for biological imaging often present themselves as black boxes whose precise operating principle remains unknown, and whose optical resolution and price seem to be in inverse proportion to each other. With UC2 (You. See. Too.) we present a low-cost, 3D-printed, open-source, modular microscopy toolbox and demonstrate its versatility by realizing a complete microscope development cycle from concept to experimental phase. The self-contained incubator-enclosed brightfield microscope monitors monocyte to macrophage cell differentiation for seven days at cellular resolution level (e.g. 2 μm). Furthermore, by including very few additional components, the geometry is transferred into a 400 Euro light sheet fluorescence microscope for volumetric observations of a transgenic Zebrafish expressing green fluorescent protein (GFP). With this, we aim to establish an open standard in optics to facilitate interfacing with various complementary platforms. By making the content and comprehensive documentation publicly available, the systems presented here lend themselves to easy and straightforward replications, modifications, and extensions.

Suggested Citation

  • Benedict Diederich & René Lachmann & Swen Carlstedt & Barbora Marsikova & Haoran Wang & Xavier Uwurukundo & Alexander S. Mosig & Rainer Heintzmann, 2020. "A versatile and customizable low-cost 3D-printed open standard for microscopic imaging," Nature Communications, Nature, vol. 11(1), pages 1-9, December.
  • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-19447-9
    DOI: 10.1038/s41467-020-19447-9
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    Cited by:

    1. Kohei Otomo & Takaki Omura & Yuki Nozawa & Steven J. Edwards & Yukihiko Sato & Yuri Saito & Shigehiro Yagishita & Hitoshi Uchida & Yuki Watakabe & Kiyotada Naitou & Rin Yanai & Naruhiko Sahara & Satos, 2024. "descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques," Nature Communications, Nature, vol. 15(1), pages 1-19, December.

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