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A single-cell atlas and lineage analysis of the adult Drosophila ovary

Author

Listed:
  • Katja Rust

    (UCSF, Department of Anatomy
    UCSF, Department of OB-GYN/RS
    Broad Center of Regeneration Medicine and Stem Cell Research)

  • Lauren E. Byrnes

    (UCSF, Department of Anatomy
    UCSF, Department of Cell and Tissue Biology)

  • Kevin Shengyang Yu

    (UCSF, Department of Otolaryngology-Head and Neck Surgery)

  • Jason S. Park

    (UCSF, Department of Otolaryngology-Head and Neck Surgery)

  • Julie B. Sneddon

    (UCSF, Department of Anatomy
    Broad Center of Regeneration Medicine and Stem Cell Research
    UCSF, Department of Cell and Tissue Biology
    UCSF, Diabetes Center)

  • Aaron D. Tward

    (UCSF, Department of Otolaryngology-Head and Neck Surgery)

  • Todd G. Nystul

    (UCSF, Department of Anatomy
    UCSF, Department of OB-GYN/RS
    Broad Center of Regeneration Medicine and Stem Cell Research)

Abstract

The Drosophila ovary is a widely used model for germ cell and somatic tissue biology. Here we use single-cell RNA-sequencing (scRNA-seq) to build a comprehensive cell atlas of the adult Drosophila ovary that contains transcriptional profiles for every major cell type in the ovary, including the germline stem cells and their niche cells, follicle stem cells, and previously undescribed subpopulations of escort cells. In addition, we identify Gal4 lines with specific expression patterns and perform lineage tracing of subpopulations of escort cells and follicle cells. We discover that a distinct subpopulation of escort cells is able to convert to follicle stem cells in response to starvation or upon genetic manipulation, including knockdown of escargot, or overactivation of mTor or Toll signalling.

Suggested Citation

  • Katja Rust & Lauren E. Byrnes & Kevin Shengyang Yu & Jason S. Park & Julie B. Sneddon & Aaron D. Tward & Todd G. Nystul, 2020. "A single-cell atlas and lineage analysis of the adult Drosophila ovary," Nature Communications, Nature, vol. 11(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-19361-0
    DOI: 10.1038/s41467-020-19361-0
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    Cited by:

    1. Yue Li & Tianfeng Lu & Pengzhen Dong & Jian Chen & Qiang Zhao & Yuying Wang & Tianheng Xiao & Honggang Wu & Quanyi Zhao & Hai Huang, 2024. "A single-cell atlas of Drosophila trachea reveals glycosylation-mediated Notch signaling in cell fate specification," Nature Communications, Nature, vol. 15(1), pages 1-18, December.

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