IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v11y2020i1d10.1038_s41467-020-19350-3.html
   My bibliography  Save this article

Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cells

Author

Listed:
  • Young Sun Hwang

    (University of Pennsylvania Perelman School of Medicine)

  • Shinnosuke Suzuki

    (University of Texas at San Antonio)

  • Yasunari Seita

    (University of Pennsylvania Perelman School of Medicine
    Bell Research Center for Reproductive Health and Cancer)

  • Jumpei Ito

    (The University of Tokyo)

  • Yuka Sakata

    (University of Pennsylvania Perelman School of Medicine)

  • Hirofumi Aso

    (The University of Tokyo)

  • Kei Sato

    (The University of Tokyo)

  • Brian P. Hermann

    (University of Texas at San Antonio)

  • Kotaro Sasaki

    (University of Pennsylvania Perelman School of Medicine)

Abstract

Establishment of spermatogonia throughout the fetal and postnatal period is essential for production of spermatozoa and male fertility. Here, we establish a protocol for in vitro reconstitution of human prospermatogonial specification whereby human primordial germ cell (PGC)-like cells differentiated from human induced pluripotent stem cells are further induced into M-prospermatogonia-like cells and T1 prospermatogonia-like cells (T1LCs) using long-term cultured xenogeneic reconstituted testes. Single cell RNA-sequencing is used to delineate the lineage trajectory leading to T1LCs, which closely resemble human T1-prospermatogonia in vivo and exhibit gene expression related to spermatogenesis and diminished proliferation, a hallmark of quiescent T1 prospermatogonia. Notably, this system enables us to visualize the dynamic and stage-specific regulation of transposable elements during human prospermatogonial specification. Together, our findings pave the way for understanding and reconstructing human male germline development in vitro.

Suggested Citation

  • Young Sun Hwang & Shinnosuke Suzuki & Yasunari Seita & Jumpei Ito & Yuka Sakata & Hirofumi Aso & Kei Sato & Brian P. Hermann & Kotaro Sasaki, 2020. "Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cells," Nature Communications, Nature, vol. 11(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-19350-3
    DOI: 10.1038/s41467-020-19350-3
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-020-19350-3
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-020-19350-3?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-19350-3. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.