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An enhanced isothermal amplification assay for viral detection

Author

Listed:
  • Jason Qian

    (Harvard Medical School
    Harvard Medical School
    Harvard Medical School)

  • Sarah A. Boswell

    (Harvard Medical School
    Harvard Medical School)

  • Christopher Chidley

    (Harvard Medical School)

  • Zhi-xiang Lu

    (Harvard Medical School
    Harvard Medical School)

  • Mary E. Pettit

    (Harvard Medical School)

  • Benjamin L. Gaudio

    (Harvard Medical School
    Harvard Medical School)

  • Jesse M. Fajnzylber

    (Harvard Medical School)

  • Ryan T. Ingram

    (Harvard Medical School)

  • Rebecca H. Ward

    (Harvard Medical School)

  • Jonathan Z. Li

    (Harvard Medical School
    Massachusetts Consortium on Pathogen Readiness)

  • Michael Springer

    (Harvard Medical School
    Harvard Medical School
    Massachusetts Consortium on Pathogen Readiness)

Abstract

Rapid, inexpensive, robust diagnostics are essential to control the spread of infectious diseases. Current state of the art diagnostics are highly sensitive and specific, but slow, and require expensive equipment. Here we report the development of a molecular diagnostic test for SARS-CoV-2 based on an enhanced recombinase polymerase amplification (eRPA) reaction. eRPA has a detection limit on patient samples down to 5 viral copies, requires minimal instrumentation, and is highly scalable and inexpensive. eRPA does not cross-react with other common coronaviruses, does not require RNA purification, and takes ~45 min from sample collection to results. eRPA represents a first step toward at-home SARS-CoV-2 detection and can be adapted to future viruses within days of genomic sequence availability.

Suggested Citation

  • Jason Qian & Sarah A. Boswell & Christopher Chidley & Zhi-xiang Lu & Mary E. Pettit & Benjamin L. Gaudio & Jesse M. Fajnzylber & Ryan T. Ingram & Rebecca H. Ward & Jonathan Z. Li & Michael Springer, 2020. "An enhanced isothermal amplification assay for viral detection," Nature Communications, Nature, vol. 11(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-19258-y
    DOI: 10.1038/s41467-020-19258-y
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