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Sensing of autoinducer-2 by functionally distinct receptors in prokaryotes

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  • Lei Zhang

    (State Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University)

  • Shuyu Li

    (State Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University)

  • Xiaozhen Liu

    (State Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University)

  • Zhuo Wang

    (State Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University)

  • Mei Jiang

    (State Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University)

  • Ruiying Wang

    (State Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University)

  • Laigong Xie

    (State Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University)

  • Qinmeng Liu

    (State Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University)

  • Xiaorong Xie

    (State Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University)

  • Daohan Shang

    (State Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University)

  • Mengyun Li

    (State Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University)

  • Zhiyan Wei

    (State Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University)

  • Yao Wang

    (State Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University)

  • Chengpeng Fan

    (School of Basic Medical Sciences, Wuhan University)

  • Zhao-Qing Luo

    (Purdue University)

  • Xihui Shen

    (State Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University)

Abstract

Autoinducer-2 (AI-2) is a quorum sensing signal that mediates communication within and between many bacterial species. However, its known receptors (LuxP and LsrB families) are not found in all the bacteria capable of responding to this signaling molecule. Here, we identify a third type of AI-2 receptor, consisting of a dCACHE domain. AI-2 binds to the dCACHE domain of chemoreceptors PctA and TlpQ of Pseudomonas aeruginosa, thus inducing chemotaxis and biofilm formation. Boron-free AI-2 is the preferred ligand for PctA and TlpQ. AI-2 also binds to the dCACHE domains of histidine kinase KinD from Bacillus subtilis and diguanylate cyclase rpHK1S-Z16 from Rhodopseudomonas palustris, enhancing their enzymatic activities. dCACHE domains (especially those belonging to a subfamily that includes the AI-2 receptors identified in the present work) are present in a large number of bacterial and archaeal proteins. Our results support the idea that AI-2 serves as a widely used signaling molecule in the coordination of cell behavior among prokaryotic species.

Suggested Citation

  • Lei Zhang & Shuyu Li & Xiaozhen Liu & Zhuo Wang & Mei Jiang & Ruiying Wang & Laigong Xie & Qinmeng Liu & Xiaorong Xie & Daohan Shang & Mengyun Li & Zhiyan Wei & Yao Wang & Chengpeng Fan & Zhao-Qing Lu, 2020. "Sensing of autoinducer-2 by functionally distinct receptors in prokaryotes," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-19243-5
    DOI: 10.1038/s41467-020-19243-5
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    Cited by:

    1. Shuyu Li & Qinmeng Liu & Chongyi Duan & Jialin Li & Hengxi Sun & Lei Xu & Qiao Yang & Yao Wang & Xihui Shen & Lei Zhang, 2023. "c-di-GMP inhibits the DNA binding activity of H-NS in Salmonella," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    2. Shuyu Li & Hengxi Sun & Jianghan Li & Yujiao Zhao & Ruiying Wang & Lei Xu & Chongyi Duan & Jialin Li & Zhuo Wang & Qinmeng Liu & Yao Wang & Songying Ouyang & Xihui Shen & Lei Zhang, 2022. "Autoinducer-2 and bile salts induce c-di-GMP synthesis to repress the T3SS via a T3SS chaperone," Nature Communications, Nature, vol. 13(1), pages 1-16, December.

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