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Identification of key sequence features required for microRNA biogenesis in plants

Author

Listed:
  • Arantxa M. L. Rojas

    (IBR (Instituto de Biología Molecular y Celular de Rosario), CONICET and Universidad Nacional de Rosario)

  • Salvador I. Drusin

    (IBR (Instituto de Biología Molecular y Celular de Rosario), CONICET and Universidad Nacional de Rosario
    Área Física, Departamento de Química-Física, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, S2002LRK Rosario)

  • Uciel Chorostecki

    (IBR (Instituto de Biología Molecular y Celular de Rosario), CONICET and Universidad Nacional de Rosario
    Barcelona Supercomputing Centre (BSC-CNS)
    Institute for Research in Biomedicine (IRB), The Barcelona Institute of Science and Technology)

  • Julieta L. Mateos

    (IBR (Instituto de Biología Molecular y Celular de Rosario), CONICET and Universidad Nacional de Rosario
    Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE), CONICET-UBA)

  • Belén Moro

    (IBR (Instituto de Biología Molecular y Celular de Rosario), CONICET and Universidad Nacional de Rosario
    Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Campus UAB)

  • Nicolas G. Bologna

    (IBR (Instituto de Biología Molecular y Celular de Rosario), CONICET and Universidad Nacional de Rosario
    Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Campus UAB)

  • Edgardo G. Bresso

    (IBR (Instituto de Biología Molecular y Celular de Rosario), CONICET and Universidad Nacional de Rosario)

  • Arnaldo Schapire

    (IBR (Instituto de Biología Molecular y Celular de Rosario), CONICET and Universidad Nacional de Rosario)

  • Rodolfo M. Rasia

    (IBR (Instituto de Biología Molecular y Celular de Rosario), CONICET and Universidad Nacional de Rosario
    Área Biofísica, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario)

  • Diego M. Moreno

    (Instituto de Química de Rosario (CONICET-UNR)
    Área Química General e Inorgánica, Departamento de Química-Física, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario)

  • Javier F. Palatnik

    (IBR (Instituto de Biología Molecular y Celular de Rosario), CONICET and Universidad Nacional de Rosario
    Centro de Estudios Interdisciplinarios, Universidad Nacional de Rosario)

Abstract

MicroRNAs (miRNAs) are endogenous small RNAs of ∼21 nt that regulate multiple biological pathways in multicellular organisms. They derive from longer transcripts that harbor an imperfect stem-loop structure. In plants, the ribonuclease type III DICER-LIKE1 assisted by accessory proteins cleaves the precursor to release the mature miRNA. Numerous studies highlight the role of the precursor secondary structure during plant miRNA biogenesis; however, little is known about the relevance of the precursor sequence. Here, we analyzed the sequence composition of plant miRNA primary transcripts and found specifically located sequence biases. We show that changes in the identity of specific nucleotides can increase or abolish miRNA biogenesis. Most conspicuously, our analysis revealed that the identity of the nucleotides at unpaired positions of the precursor plays a crucial role during miRNA biogenesis in Arabidopsis.

Suggested Citation

  • Arantxa M. L. Rojas & Salvador I. Drusin & Uciel Chorostecki & Julieta L. Mateos & Belén Moro & Nicolas G. Bologna & Edgardo G. Bresso & Arnaldo Schapire & Rodolfo M. Rasia & Diego M. Moreno & Javier , 2020. "Identification of key sequence features required for microRNA biogenesis in plants," Nature Communications, Nature, vol. 11(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-19129-6
    DOI: 10.1038/s41467-020-19129-6
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