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21-nt phasiRNAs direct target mRNA cleavage in rice male germ cells

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  • Pengfei Jiang

    (Center for Plant Biology, School of Life Sciences, Tsinghua University
    Tsinghua-Peking Center for Life Sciences)

  • Bi Lian

    (Center for Plant Biology, School of Life Sciences, Tsinghua University
    Tsinghua-Peking Center for Life Sciences)

  • Changzhen Liu

    (State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences)

  • Zeyu Fu

    (Center for Plant Biology, School of Life Sciences, Tsinghua University
    Tsinghua-Peking Center for Life Sciences)

  • Yi Shen

    (State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences)

  • Zhukuan Cheng

    (State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences)

  • Yijun Qi

    (Center for Plant Biology, School of Life Sciences, Tsinghua University
    Tsinghua-Peking Center for Life Sciences)

Abstract

In grasses, phased small interfering RNAs (phasiRNAs), 21- or 24-nucleotide (nt) in length, are predominantly expressed in anthers and play a role in regulating male fertility. However, their targets and mode of action on the targets remain unknown. Here we profile phasiRNA expression in premeiotic and meiotic spikelets as well as in purified male meiocytes at early prophase I, tetrads and microspores in rice. We show that 21-nt phasiRNAs are most abundant in meiocytes at early prophase I while 24-nt phasiRNAs are more abundant in tetrads and microspores. By performing highly sensitive degradome sequencing, we find that 21-nt phasiRNAs direct target mRNA cleavage in male germ cells, especially in meiocytes at early prophase I. These targets include 435 protein-coding genes and 71 transposons that show an enrichment for carbohydrate biosynthetic and metabolic pathways. Our study provides strong evidence that 21-nt phasiRNAs act in a target-cleavage mode and may facilitate the progression of meiosis by fine-tuning carbohydrate biosynthesis and metabolism in male germ cells.

Suggested Citation

  • Pengfei Jiang & Bi Lian & Changzhen Liu & Zeyu Fu & Yi Shen & Zhukuan Cheng & Yijun Qi, 2020. "21-nt phasiRNAs direct target mRNA cleavage in rice male germ cells," Nature Communications, Nature, vol. 11(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-19034-y
    DOI: 10.1038/s41467-020-19034-y
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    Cited by:

    1. Hinako Tamotsu & Koji Koizumi & Alejandro Villar Briones & Reina Komiya, 2023. "Spatial distribution of three ARGONAUTEs regulates the anther phasiRNA pathway," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    2. Xueting Gu & Fuyan Si & Zhengxiang Feng & Shunjie Li & Di Liang & Pei Yang & Chao Yang & Bin Yan & Jun Tang & Yu Yang & Tai Li & Lin Li & Jinling Zhou & Ji Li & Lili Feng & Ji-Yun Liu & Yuanzhu Yang &, 2023. "The OsSGS3-tasiRNA-OsARF3 module orchestrates abiotic-biotic stress response trade-off in rice," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    3. Fuxi Rong & Yusong Lv & Pingchuan Deng & Xia Wu & Yaqi Zhang & Erkui Yue & Yuxin Shen & Sajid Muhammad & Fangrui Ni & Hongwu Bian & Xiangjin Wei & Weijun Zhou & Peisong Hu & Liang Wu, 2024. "Switching action modes of miR408-5p mediates auxin signaling in rice," Nature Communications, Nature, vol. 15(1), pages 1-17, December.

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