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A fast impedance-based antimicrobial susceptibility test

Author

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  • Daniel C. Spencer

    (University of Southampton)

  • Teagan F. Paton

    (PathWest Laboratory Medicine)

  • Kieran T. Mulroney

    (University of Western Australia)

  • Timothy J. J. Inglis

    (PathWest Laboratory Medicine
    University of Western Australia)

  • J. Mark Sutton

    (National Infection Service)

  • Hywel Morgan

    (University of Southampton)

Abstract

There is an urgent need to develop simple and fast antimicrobial susceptibility tests (ASTs) that allow informed prescribing of antibiotics. Here, we describe a label-free AST that can deliver results within an hour, using an actively dividing culture as starting material. The bacteria are incubated in the presence of an antibiotic for 30 min, and then approximately 105 cells are analysed one-by-one with microfluidic impedance cytometry for 2–3 min. The measured electrical characteristics reflect the phenotypic response of the bacteria to the mode of action of a particular antibiotic, in a 30-minute incubation window. The results are consistent with those obtained by classical broth microdilution assays for a range of antibiotics and bacterial species.

Suggested Citation

  • Daniel C. Spencer & Teagan F. Paton & Kieran T. Mulroney & Timothy J. J. Inglis & J. Mark Sutton & Hywel Morgan, 2020. "A fast impedance-based antimicrobial susceptibility test," Nature Communications, Nature, vol. 11(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-18902-x
    DOI: 10.1038/s41467-020-18902-x
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