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Structural basis for the transition from translation initiation to elongation by an 80S-eIF5B complex

Author

Listed:
  • Jinfan Wang

    (Stanford University School of Medicine)

  • Jing Wang

    (Columbia University)

  • Byung-Sik Shin

    (Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH)

  • Joo-Ran Kim

    (Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH)

  • Thomas E. Dever

    (Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH)

  • Joseph D. Puglisi

    (Stanford University School of Medicine)

  • Israel S. Fernández

    (Columbia University)

Abstract

Recognition of a start codon by the initiator aminoacyl-tRNA determines the reading frame of messenger RNA (mRNA) translation by the ribosome. In eukaryotes, the GTPase eIF5B collaborates in the correct positioning of the initiator Met-tRNAiMet on the ribosome in the later stages of translation initiation, gating entrance into elongation. Leveraging the long residence time of eIF5B on the ribosome recently identified by single-molecule fluorescence measurements, we determine the cryoEM structure of the naturally long-lived ribosome complex with eIF5B and Met-tRNAiMet immediately before transition into elongation. The structure uncovers an unexpected, eukaryotic specific and dynamic fidelity checkpoint implemented by eIF5B in concert with components of the large ribosomal subunit.

Suggested Citation

  • Jinfan Wang & Jing Wang & Byung-Sik Shin & Joo-Ran Kim & Thomas E. Dever & Joseph D. Puglisi & Israel S. Fernández, 2020. "Structural basis for the transition from translation initiation to elongation by an 80S-eIF5B complex," Nature Communications, Nature, vol. 11(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-18829-3
    DOI: 10.1038/s41467-020-18829-3
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    Cited by:

    1. Runlai Hang & Hao Li & Wenjing Liu & Runyu Wang & Hao Hu & Meng Chen & Chenjiang You & Xuemei Chen, 2024. "HOT3/eIF5B1 confers Kozak motif-dependent translational control of photosynthesis-associated nuclear genes for chloroplast biogenesis," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    2. Daniel Sultanov & Andreas Hochwagen, 2022. "Varying strength of selection contributes to the intragenomic diversity of rRNA genes," Nature Communications, Nature, vol. 13(1), pages 1-14, December.

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