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Tailoring poplar lignin without yield penalty by combining a null and haploinsufficient CINNAMOYL-CoA REDUCTASE2 allele

Author

Listed:
  • Barbara De Meester

    (Department of Plant Biotechnology and Bioinformatics, Ghent University
    VIB Center for Plant Systems Biology)

  • Barbara Madariaga Calderón

    (Department of Plant Biotechnology and Bioinformatics, Ghent University
    VIB Center for Plant Systems Biology)

  • Lisanne de Vries

    (Department of Plant Biotechnology and Bioinformatics, Ghent University
    VIB Center for Plant Systems Biology)

  • Jacob Pollier

    (VIB Metabolomics Core)

  • Geert Goeminne

    (VIB Metabolomics Core)

  • Jan Van Doorsselaere

    (Higher Institute for Nursing and Biotechnology, VIVES University College)

  • Mingjie Chen

    (University of Wisconsin-Madison
    US Department of Energy, Great Lakes Bioenergy Research Center, Wisconsin Energy Institute)

  • John Ralph

    (University of Wisconsin-Madison
    US Department of Energy, Great Lakes Bioenergy Research Center, Wisconsin Energy Institute)

  • Ruben Vanholme

    (Department of Plant Biotechnology and Bioinformatics, Ghent University
    VIB Center for Plant Systems Biology)

  • Wout Boerjan

    (Department of Plant Biotechnology and Bioinformatics, Ghent University
    VIB Center for Plant Systems Biology)

Abstract

Lignin causes lignocellulosic biomass recalcitrance to enzymatic hydrolysis. Engineered low-lignin plants have reduced recalcitrance but often exhibit yield penalties, offsetting their gains in fermentable sugar yield. Here, CRISPR/Cas9-generated CCR2(−/*) line 12 poplars have one knockout CCR2 allele while the other contains a 3-bp deletion, resulting in a 114I115A-to-114T conversion in the corresponding protein. Despite having 10% less lignin, CCR2(−/*) line 12 grows normally. On a plant basis, the saccharification efficiency of CCR2(−/*) line 12 is increased by 25–41%, depending on the pretreatment. Analysis of monoallelic CCR2 knockout lines shows that the reduced lignin amount in CCR2(−/*) line 12 is due to the combination of a null and the specific haploinsufficient CCR2 allele. Analysis of another CCR2(−/*) line shows that depending on the specific CCR2 amino-acid change, lignin amount and growth can be affected to different extents. Our findings open up new possibilities for stably fine-tuning residual gene function in planta.

Suggested Citation

  • Barbara De Meester & Barbara Madariaga Calderón & Lisanne de Vries & Jacob Pollier & Geert Goeminne & Jan Van Doorsselaere & Mingjie Chen & John Ralph & Ruben Vanholme & Wout Boerjan, 2020. "Tailoring poplar lignin without yield penalty by combining a null and haploinsufficient CINNAMOYL-CoA REDUCTASE2 allele," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-18822-w
    DOI: 10.1038/s41467-020-18822-w
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