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Optoribogenetic control of regulatory RNA molecules

Author

Listed:
  • Sebastian Pilsl

    (University of Bonn)

  • Charles Morgan

    (University of Bonn)

  • Moujab Choukeife

    (University of Bonn)

  • Andreas Möglich

    (University of Bayreuth)

  • Günter Mayer

    (University of Bonn
    University of Bonn)

Abstract

Short regulatory RNA molecules underpin gene expression and govern cellular state and physiology. To establish an alternative layer of control over these processes, we generated chimeric regulatory RNAs that interact reversibly and light-dependently with the light-oxygen-voltage photoreceptor PAL. By harnessing this interaction, the function of micro RNAs (miRs) and short hairpin (sh) RNAs in mammalian cells can be regulated in a spatiotemporally precise manner. The underlying strategy is generic and can be adapted to near-arbitrary target sequences. Owing to full genetic encodability, it establishes optoribogenetic control of cell state and physiology. The method stands to facilitate the non-invasive, reversible and spatiotemporally resolved study of regulatory RNAs and protein function in cellular and organismal environments.

Suggested Citation

  • Sebastian Pilsl & Charles Morgan & Moujab Choukeife & Andreas Möglich & Günter Mayer, 2020. "Optoribogenetic control of regulatory RNA molecules," Nature Communications, Nature, vol. 11(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-18673-5
    DOI: 10.1038/s41467-020-18673-5
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    Cited by:

    1. Kumar, Premashis & Banerjee, Kinshuk & Gangopadhyay, Gautam, 2022. "Interplay of energy, dissipation, and error in kinetic proofreading: Control via concentration and binding energy," Physica A: Statistical Mechanics and its Applications, Elsevier, vol. 603(C).

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