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T-Plastin reinforces membrane protrusions to bridge matrix gaps during cell migration

Author

Listed:
  • Damien Garbett

    (Stanford University)

  • Anjali Bisaria

    (Stanford University)

  • Changsong Yang

    (University of Pennsylvania)

  • Dannielle G. McCarthy

    (Stanford University)

  • Arnold Hayer

    (Stanford University
    McGill University)

  • W. E. Moerner

    (Stanford University)

  • Tatyana M. Svitkina

    (University of Pennsylvania)

  • Tobias Meyer

    (Stanford University
    Weill Cornell Medicine)

Abstract

Migrating cells move across diverse assemblies of extracellular matrix (ECM) that can be separated by micron-scale gaps. For membranes to protrude and reattach across a gap, actin filaments, which are relatively weak as single filaments, must polymerize outward from adhesion sites to push membranes towards distant sites of new adhesion. Here, using micropatterned ECMs, we identify T-Plastin, one of the most ancient actin bundling proteins, as an actin stabilizer that promotes membrane protrusions and enables bridging of ECM gaps. We show that T-Plastin widens and lengthens protrusions and is specifically enriched in active protrusions where F-actin is devoid of non-muscle myosin II activity. Together, our study uncovers critical roles of the actin bundler T-Plastin to promote protrusions and migration when adhesion is spatially-gapped.

Suggested Citation

  • Damien Garbett & Anjali Bisaria & Changsong Yang & Dannielle G. McCarthy & Arnold Hayer & W. E. Moerner & Tatyana M. Svitkina & Tobias Meyer, 2020. "T-Plastin reinforces membrane protrusions to bridge matrix gaps during cell migration," Nature Communications, Nature, vol. 11(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-18586-3
    DOI: 10.1038/s41467-020-18586-3
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    Cited by:

    1. Antoine A. Khalil & Daan Smits & Peter D. Haughton & Thijs Koorman & Karin A. Jansen & Mathijs P. Verhagen & Mirjam Net & Kitty Zwieten & Lotte Enserink & Lisa Jansen & Abdelrahman G. El-Gammal & Daan, 2024. "A YAP-centered mechanotransduction loop drives collective breast cancer cell invasion," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    2. Franziska Lehne & Thomas Pokrant & Sabnam Parbin & Gabriela Salinas & Jörg Großhans & Katja Rust & Jan Faix & Sven Bogdan, 2022. "Calcium bursts allow rapid reorganization of EFhD2/Swip-1 cross-linked actin networks in epithelial wound closure," Nature Communications, Nature, vol. 13(1), pages 1-17, December.

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