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Surfaceome dynamics reveal proteostasis-independent reorganization of neuronal surface proteins during development and synaptic plasticity

Author

Listed:
  • Marc Oostrum

    (Neuroscience Center Zurich
    ETH Zurich
    Swiss Institute of Bioinformatics (SIB))

  • Benjamin Campbell

    (Neuroscience Center Zurich
    University of Zurich)

  • Charlotte Seng

    (Neuroscience Center Zurich
    University of Zurich)

  • Maik Müller

    (ETH Zurich
    Swiss Institute of Bioinformatics (SIB))

  • Susanne Dieck

    (Max Planck Institute for Brain Research)

  • Jacqueline Hammer

    (ETH Zurich)

  • Patrick G. A. Pedrioli

    (ETH Zurich
    Swiss Institute of Bioinformatics (SIB))

  • Csaba Földy

    (Neuroscience Center Zurich
    University of Zurich)

  • Shiva K. Tyagarajan

    (Neuroscience Center Zurich
    University of Zurich)

  • Bernd Wollscheid

    (Neuroscience Center Zurich
    ETH Zurich
    Swiss Institute of Bioinformatics (SIB))

Abstract

Neurons are highly compartmentalized cells with tightly controlled subcellular protein organization. While brain transcriptome, connectome and global proteome maps are being generated, system-wide analysis of temporal protein dynamics at the subcellular level are currently lacking. Here, we perform a temporally-resolved surfaceome analysis of primary neuron cultures and reveal dynamic surface protein clusters that reflect the functional requirements during distinct stages of neuronal development. Direct comparison of surface and total protein pools during development and homeostatic synaptic scaling demonstrates system-wide proteostasis-independent remodeling of the neuronal surface, illustrating widespread regulation on the level of surface trafficking. Finally, quantitative analysis of the neuronal surface during chemical long-term potentiation (cLTP) reveals fast externalization of diverse classes of surface proteins beyond the AMPA receptor, providing avenues to investigate the requirement of exocytosis for LTP. Our resource (neurosurfaceome.ethz.ch) highlights the importance of subcellular resolution for systems-level understanding of cellular processes.

Suggested Citation

  • Marc Oostrum & Benjamin Campbell & Charlotte Seng & Maik Müller & Susanne Dieck & Jacqueline Hammer & Patrick G. A. Pedrioli & Csaba Földy & Shiva K. Tyagarajan & Bernd Wollscheid, 2020. "Surfaceome dynamics reveal proteostasis-independent reorganization of neuronal surface proteins during development and synaptic plasticity," Nature Communications, Nature, vol. 11(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-18494-6
    DOI: 10.1038/s41467-020-18494-6
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    Cited by:

    1. Nathan Bénac & G. Ezequiel Saraceno & Corey Butler & Nahoko Kuga & Yuya Nishimura & Taiki Yokoi & Ping Su & Takuya Sasaki & Mar Petit-Pedrol & Rémi Galland & Vincent Studer & Fang Liu & Yuji Ikegaya &, 2024. "Non-canonical interplay between glutamatergic NMDA and dopamine receptors shapes synaptogenesis," Nature Communications, Nature, vol. 15(1), pages 1-19, December.
    2. Ana Martinez-Val & Dorte B. Bekker-Jensen & Sophia Steigerwald & Claire Koenig & Ole Østergaard & Adi Mehta & Trung Tran & Krzysztof Sikorski & Estefanía Torres-Vega & Ewa Kwasniewicz & Sólveig Hlín B, 2021. "Spatial-proteomics reveals phospho-signaling dynamics at subcellular resolution," Nature Communications, Nature, vol. 12(1), pages 1-17, December.

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