IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v11y2020i1d10.1038_s41467-020-18289-9.html
   My bibliography  Save this article

Transgenerational inheritance of impaired larval T cell development in zebrafish

Author

Listed:
  • Norimasa Iwanami

    (Department of Developmental Immunology, Max Planck Institute of Immunobiology and Epigenetics
    Utsunomiya University)

  • Divine-Fondzenyuy Lawir

    (Department of Developmental Immunology, Max Planck Institute of Immunobiology and Epigenetics
    University of Cologne)

  • Katarzyna Sikora

    (Department of Developmental Immunology, Max Planck Institute of Immunobiology and Epigenetics)

  • Connor O´Meara

    (Department of Developmental Immunology, Max Planck Institute of Immunobiology and Epigenetics)

  • Kohei Takeshita

    (RIKEN SPring-8 Center, Sayo)

  • Michael Schorpp

    (Department of Developmental Immunology, Max Planck Institute of Immunobiology and Epigenetics)

  • Thomas Boehm

    (Department of Developmental Immunology, Max Planck Institute of Immunobiology and Epigenetics)

Abstract

Evidence for transgenerational inheritance of epigenetic information in vertebrates is scarce. Aberrant patterns of DNA methylation in gametes may set the stage for transmission into future generations. Here, we describe a viable hypomorphic allele of dnmt1 in zebrafish that causes widespread demethylation of CpG dinucleotides in sperm and somatic tissues. We find that homozygous mutants are essentially normal, with the exception of drastically impaired lymphopoiesis, affecting both larval and adult phases of T cell development. The phenotype of impaired larval (but not adult) T cell development is transmitted to subsequent generations by genotypically wildtype fish. We further find that about 200 differentially methylated regions in sperm DNA of transmitting and non-transmitting males, including hypermethylated sites associated with runx3 and rptor genes, whose reduced activities are associated with impaired larval T cell development. Our results indicate a particular sensitivity of larval T cell development to transgenerationally inherited epimutations.

Suggested Citation

  • Norimasa Iwanami & Divine-Fondzenyuy Lawir & Katarzyna Sikora & Connor O´Meara & Kohei Takeshita & Michael Schorpp & Thomas Boehm, 2020. "Transgenerational inheritance of impaired larval T cell development in zebrafish," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-18289-9
    DOI: 10.1038/s41467-020-18289-9
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-020-18289-9
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-020-18289-9?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Donna M. Bond & Oscar Ortega-Recalde & Melanie K. Laird & Takashi Hayakawa & Kyle S. Richardson & Finlay.C. B. Reese & Bruce Kyle & Brooke E. McIsaac-Williams & Bruce C. Robertson & Yolanda Heezik & A, 2023. "The admixed brushtail possum genome reveals invasion history in New Zealand and novel imprinted genes," Nature Communications, Nature, vol. 14(1), pages 1-17, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-18289-9. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.