IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v11y2020i1d10.1038_s41467-020-18261-7.html
   My bibliography  Save this article

Functional regulation of an ancestral RAG transposon ProtoRAG by a trans-acting factor YY1 in lancelet

Author

Listed:
  • Song Liu

    (School of Life Sciences, Sun Yat-sen University)

  • Shaochun Yuan

    (School of Life Sciences, Sun Yat-sen University
    Qingdao National Laboratory for Marine Science and Technology)

  • Xiaoman Gao

    (School of Life Sciences, Sun Yat-sen University)

  • Xin Tao

    (School of Life Sciences, Sun Yat-sen University)

  • Wenjuan Yu

    (School of Life Sciences, Sun Yat-sen University)

  • Xu Li

    (School of Life Sciences, Sun Yat-sen University)

  • Shangwu Chen

    (School of Life Sciences, Sun Yat-sen University)

  • Anlong Xu

    (School of Life Sciences, Sun Yat-sen University
    Beijing University of Chinese Medicine)

Abstract

The discovery of ancestral RAG transposons in early deuterostomia reveals the origin of vertebrate V(D)J recombination. Here, we analyze the functional regulation of a RAG transposon, ProtoRAG, in lancelet. We find that a specific interaction between the cis-acting element within the TIR sequences of ProtoRAG and a trans-acting factor, lancelet YY1-like (bbYY1), is important for the transcriptional regulation of lancelet RAG-like genes (bbRAG1L and bbRAG2L). Mechanistically, bbYY1 suppresses the transposition of ProtoRAG; meanwhile, bbYY1 promotes host DNA rejoins (HDJ) and TIR-TIR joints (TTJ) after TIR-dependent excision by facilitating the binding of bbRAG1L/2 L to TIR-containing DNA, and by interacting with the bbRAG1L/2 L complex. Our data thus suggest that bbYY1 has dual functions in fine-tuning the activity of ProtoRAG and maintaining the genome stability of the host.

Suggested Citation

  • Song Liu & Shaochun Yuan & Xiaoman Gao & Xin Tao & Wenjuan Yu & Xu Li & Shangwu Chen & Anlong Xu, 2020. "Functional regulation of an ancestral RAG transposon ProtoRAG by a trans-acting factor YY1 in lancelet," Nature Communications, Nature, vol. 11(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-18261-7
    DOI: 10.1038/s41467-020-18261-7
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-020-18261-7
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-020-18261-7?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-18261-7. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.