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Fast and multiplexed superresolution imaging with DNA-PAINT-ERS

Author

Listed:
  • Fehmi Civitci

    (Oregon Health and Science University)

  • Julia Shangguan

    (Oregon Health and Science University
    Oregon Health and Science University)

  • Ting Zheng

    (Oregon Health and Science University)

  • Kai Tao

    (Oregon Health and Science University
    Oregon Health and Science University)

  • Matthew Rames

    (Oregon Health and Science University
    Oregon Health and Science University)

  • John Kenison

    (Oregon Health and Science University)

  • Ying Zhang

    (Oregon Health and Science University
    Oregon Health and Science University)

  • Lei Wu

    (Oregon Health and Science University
    Wuhan University)

  • Carey Phelps

    (Oregon Health and Science University
    Oregon Health and Science University)

  • Sadik Esener

    (Oregon Health and Science University
    Oregon Health and Science University)

  • Xiaolin Nan

    (Oregon Health and Science University
    Oregon Health and Science University
    Oregon Health and Science University)

Abstract

DNA points accumulation for imaging in nanoscale topography (DNA-PAINT) facilitates multiplexing in superresolution microscopy but is practically limited by slow imaging speed. To address this issue, we propose the additions of ethylene carbonate (EC) to the imaging buffer, sequence repeats to the docking strand, and a spacer between the docking strand and the affinity agent. Collectively termed DNA-PAINT-ERS (E = EC, R = Repeating sequence, and S = Spacer), these strategies can be easily integrated into current DNA-PAINT workflows for both accelerated imaging speed and improved image quality through optimized DNA hybridization kinetics and efficiency. We demonstrate the general applicability of DNA-PAINT-ERS for fast, multiplexed superresolution imaging using previously validated oligonucleotide constructs with slight modifications.

Suggested Citation

  • Fehmi Civitci & Julia Shangguan & Ting Zheng & Kai Tao & Matthew Rames & John Kenison & Ying Zhang & Lei Wu & Carey Phelps & Sadik Esener & Xiaolin Nan, 2020. "Fast and multiplexed superresolution imaging with DNA-PAINT-ERS," Nature Communications, Nature, vol. 11(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-18181-6
    DOI: 10.1038/s41467-020-18181-6
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    Cited by:

    1. Kaarjel K. Narayanasamy & Johanna V. Rahm & Siddharth Tourani & Mike Heilemann, 2022. "Fast DNA-PAINT imaging using a deep neural network," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    2. Di-Ao Liu & Kai Tao & Bin Wu & Ziyan Yu & Malwina Szczepaniak & Matthew Rames & Changsong Yang & Tatyana Svitkina & Yueyao Zhu & Fengyuan Xu & Xiaolin Nan & Wei Guo, 2023. "A phosphoinositide switch mediates exocyst recruitment to multivesicular endosomes for exosome secretion," Nature Communications, Nature, vol. 14(1), pages 1-16, December.

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