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A panel of human neutralizing mAbs targeting SARS-CoV-2 spike at multiple epitopes

Author

Listed:
  • Tal Noy-Porat

    (Israel Institute for Biological Research)

  • Efi Makdasi

    (Israel Institute for Biological Research)

  • Ron Alcalay

    (Israel Institute for Biological Research)

  • Adva Mechaly

    (Israel Institute for Biological Research)

  • Yinon Levy

    (Israel Institute for Biological Research)

  • Adi Bercovich-Kinori

    (Israel Institute for Biological Research)

  • Ayelet Zauberman

    (Israel Institute for Biological Research)

  • Hadas Tamir

    (Israel Institute for Biological Research)

  • Yfat Yahalom-Ronen

    (Israel Institute for Biological Research)

  • Ma’ayan Israeli

    (Israel Institute for Biological Research)

  • Eyal Epstein

    (Israel Institute for Biological Research)

  • Hagit Achdout

    (Israel Institute for Biological Research)

  • Sharon Melamed

    (Israel Institute for Biological Research)

  • Theodor Chitlaru

    (Israel Institute for Biological Research)

  • Shay Weiss

    (Israel Institute for Biological Research)

  • Eldar Peretz

    (Israel Institute for Biological Research)

  • Osnat Rosen

    (Israel Institute for Biological Research)

  • Nir Paran

    (Israel Institute for Biological Research)

  • Shmuel Yitzhaki

    (Israel Institute for Biological Research)

  • Shmuel C. Shapira

    (Israel Institute for Biological Research)

  • Tomer Israely

    (Israel Institute for Biological Research)

  • Ohad Mazor

    (Israel Institute for Biological Research)

  • Ronit Rosenfeld

    (Israel Institute for Biological Research)

Abstract

The novel highly transmissible human coronavirus SARS-CoV-2 is the causative agent of the COVID-19 pandemic. Thus far, there is no approved therapeutic drug specifically targeting this emerging virus. Here we report the isolation and characterization of a panel of human neutralizing monoclonal antibodies targeting the SARS-CoV-2 receptor binding domain (RBD). These antibodies were selected from a phage display library constructed using peripheral circulatory lymphocytes collected from patients at the acute phase of the disease. These neutralizing antibodies are shown to recognize distinct epitopes on the viral spike RBD. A subset of the antibodies exert their inhibitory activity by abrogating binding of the RBD to the human ACE2 receptor. The human monoclonal antibodies described here represent a promising basis for the design of efficient combined post-exposure therapy for SARS-CoV-2 infection.

Suggested Citation

  • Tal Noy-Porat & Efi Makdasi & Ron Alcalay & Adva Mechaly & Yinon Levy & Adi Bercovich-Kinori & Ayelet Zauberman & Hadas Tamir & Yfat Yahalom-Ronen & Ma’ayan Israeli & Eyal Epstein & Hagit Achdout & Sh, 2020. "A panel of human neutralizing mAbs targeting SARS-CoV-2 spike at multiple epitopes," Nature Communications, Nature, vol. 11(1), pages 1-7, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-18159-4
    DOI: 10.1038/s41467-020-18159-4
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    Cited by:

    1. Andrew M. King & Daniel A. Anderson & Emerson Glassey & Thomas H. Segall-Shapiro & Zhengan Zhang & David L. Niquille & Amanda C. Embree & Katelin Pratt & Thomas L. Williams & D. Benjamin Gordon & Chri, 2021. "Selection for constrained peptides that bind to a single target protein," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    2. Romain Rouet & Jake Y. Henry & Matt D. Johansen & Meghna Sobti & Harikrishnan Balachandran & David B. Langley & Gregory J. Walker & Helen Lenthall & Jennifer Jackson & Stephanie Ubiparipovic & Ohan Ma, 2023. "Broadly neutralizing SARS-CoV-2 antibodies through epitope-based selection from convalescent patients," Nature Communications, Nature, vol. 14(1), pages 1-13, December.

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