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Rational design of DNA nanostructures for single molecule biosensing

Author

Listed:
  • Mukhil Raveendran

    (University of Leeds)

  • Andrew J. Lee

    (University of Leeds
    University of Leeds)

  • Rajan Sharma

    (University of Leeds)

  • Christoph Wälti

    (University of Leeds
    University of Leeds)

  • Paolo Actis

    (University of Leeds
    University of Leeds)

Abstract

The ability to detect low concentrations of biomarkers in patient samples is one of the cornerstones of modern healthcare. In general, biosensing approaches are based on measuring signals resulting from the interaction of a large ensemble of molecules with the sensor. Here, we report a biosensor platform using DNA origami featuring a central cavity with a target-specific DNA aptamer coupled with a nanopore read-out to enable individual biomarker detection. We show that the modulation of the ion current through the nanopore upon the DNA origami translocation strongly depends on the presence of the biomarker in the cavity. We exploit this to generate a biosensing platform with a limit of detection of 3 nM and capable of the detection of human C-reactive protein (CRP) in clinically relevant fluids. Future development of this approach may enable multiplexed biomarker detection by using ribbons of DNA origami with integrated barcoding.

Suggested Citation

  • Mukhil Raveendran & Andrew J. Lee & Rajan Sharma & Christoph Wälti & Paolo Actis, 2020. "Rational design of DNA nanostructures for single molecule biosensing," Nature Communications, Nature, vol. 11(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-18132-1
    DOI: 10.1038/s41467-020-18132-1
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    Cited by:

    1. Ren Ren & Shenglin Cai & Xiaona Fang & Xiaoyi Wang & Zheng Zhang & Micol Damiani & Charlotte Hudlerova & Annachiara Rosa & Joshua Hope & Nicola J. Cook & Peter Gorelkin & Alexander Erofeev & Pavel Nov, 2023. "Multiplexed detection of viral antigen and RNA using nanopore sensing and encoded molecular probes," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    2. Chalmers C. Chau & Christopher M. Maffeo & Aleksei Aksimentiev & Sheena E. Radford & Eric W. Hewitt & Paolo Actis, 2024. "Single molecule delivery into living cells," Nature Communications, Nature, vol. 15(1), pages 1-11, December.

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