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DEPS-1 is required for piRNA-dependent silencing and PIWI condensate organisation in Caenorhabditis elegans

Author

Listed:
  • Kin Man Suen

    (University of Cambridge
    University of Cambridge)

  • Fabian Braukmann

    (University of Cambridge
    University of Cambridge)

  • Richard Butler

    (University of Cambridge)

  • Dalila Bensaddek

    (University of Dundee
    King Abdullah University of Science and Technology)

  • Alper Akay

    (University of Cambridge
    University of Cambridge
    University of East Anglia)

  • Chi-Chuan Lin

    (University of Leeds, LC Miall Building)

  • Dovilė Milonaitytė

    (University of Leeds, LC Miall Building)

  • Neel Doshi

    (University of Cambridge, School of Clinical Medicine, Cambridge Biomedical Campus)

  • Alexandra Sapetschnig

    (Wellcome Trust Genome Campus)

  • Angus Lamond

    (University of Dundee)

  • John Edward Ladbury

    (University of Leeds, LC Miall Building)

  • Eric Alexander Miska

    (University of Cambridge
    University of Cambridge
    Wellcome Trust Genome Campus)

Abstract

Membraneless organelles are sites for RNA biology including small non-coding RNA (ncRNA) mediated gene silencing. How small ncRNAs utilise phase separated environments for their function is unclear. We investigated how the PIWI-interacting RNA (piRNA) pathway engages with the membraneless organelle P granule in Caenorhabditis elegans. Proteomic analysis of the PIWI protein PRG-1 reveals an interaction with the constitutive P granule protein DEPS-1. DEPS-1 is not required for piRNA biogenesis but piRNA-dependent silencing: deps-1 mutants fail to produce the secondary endo-siRNAs required for the silencing of piRNA targets. We identify a motif on DEPS-1 which mediates a direct interaction with PRG-1. DEPS-1 and PRG-1 form intertwining clusters to build elongated condensates in vivo which are dependent on the Piwi-interacting motif of DEPS-1. Additionally, we identify EDG-1 as an interactor of DEPS-1 and PRG-1. Our study reveals how specific protein-protein interactions drive the spatial organisation and piRNA-dependent silencing within membraneless organelles.

Suggested Citation

  • Kin Man Suen & Fabian Braukmann & Richard Butler & Dalila Bensaddek & Alper Akay & Chi-Chuan Lin & Dovilė Milonaitytė & Neel Doshi & Alexandra Sapetschnig & Angus Lamond & John Edward Ladbury & Eric A, 2020. "DEPS-1 is required for piRNA-dependent silencing and PIWI condensate organisation in Caenorhabditis elegans," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-18089-1
    DOI: 10.1038/s41467-020-18089-1
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    Cited by:

    1. Wenjun Chen & Jordan S. Brown & Tao He & Wei-Sheng Wu & Shikui Tu & Zhiping Weng & Donglei Zhang & Heng-Chi Lee, 2022. "GLH/VASA helicases promote germ granule formation to ensure the fidelity of piRNA-mediated transcriptome surveillance," Nature Communications, Nature, vol. 13(1), pages 1-14, December.

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