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Cytokinin fluoroprobe reveals multiple sites of cytokinin perception at plasma membrane and endoplasmic reticulum

Author

Listed:
  • Karolina Kubiasová

    (Palacký University)

  • Juan Carlos Montesinos

    (Institute of Science and Technology (IST))

  • Olga Šamajová

    (Palacký University)

  • Jaroslav Nisler

    (Palacký University
    Institute of Experimental Botany of the Czech Academy of Sciences and Faculty of Science of Palacký University)

  • Václav Mik

    (Palacký University)

  • Hana Semerádová

    (Institute of Science and Technology (IST))

  • Lucie Plíhalová

    (Palacký University
    Institute of Experimental Botany of the Czech Academy of Sciences and Faculty of Science of Palacký University)

  • Ondřej Novák

    (Institute of Experimental Botany of the Czech Academy of Sciences and Faculty of Science of Palacký University)

  • Peter Marhavý

    (Institute of Science and Technology (IST)
    Swedish University of Agricultural Sciences)

  • Nicola Cavallari

    (Institute of Science and Technology (IST))

  • David Zalabák

    (Palacký University)

  • Karel Berka

    (Palacký University)

  • Karel Doležal

    (Palacký University
    Institute of Experimental Botany of the Czech Academy of Sciences and Faculty of Science of Palacký University)

  • Petr Galuszka
  • Jozef Šamaj

    (Palacký University)

  • Miroslav Strnad

    (Institute of Experimental Botany of the Czech Academy of Sciences and Faculty of Science of Palacký University)

  • Eva Benková

    (Institute of Science and Technology (IST))

  • Ondřej Plíhal

    (Palacký University
    Palacký University
    Institute of Experimental Botany of the Czech Academy of Sciences and Faculty of Science of Palacký University)

  • Lukáš Spíchal

    (Palacký University)

Abstract

Plant hormone cytokinins are perceived by a subfamily of sensor histidine kinases (HKs), which via a two-component phosphorelay cascade activate transcriptional responses in the nucleus. Subcellular localization of the receptors proposed the endoplasmic reticulum (ER) membrane as a principal cytokinin perception site, while study of cytokinin transport pointed to the plasma membrane (PM)-mediated cytokinin signalling. Here, by detailed monitoring of subcellular localizations of the fluorescently labelled natural cytokinin probe and the receptor ARABIDOPSIS HISTIDINE KINASE 4 (CRE1/AHK4) fused to GFP reporter, we show that pools of the ER-located cytokinin receptors can enter the secretory pathway and reach the PM in cells of the root apical meristem, and the cell plate of dividing meristematic cells. Brefeldin A (BFA) experiments revealed vesicular recycling of the receptor and its accumulation in BFA compartments. We provide a revised view on cytokinin signalling and the possibility of multiple sites of perception at PM and ER.

Suggested Citation

  • Karolina Kubiasová & Juan Carlos Montesinos & Olga Šamajová & Jaroslav Nisler & Václav Mik & Hana Semerádová & Lucie Plíhalová & Ondřej Novák & Peter Marhavý & Nicola Cavallari & David Zalabák & Karel, 2020. "Cytokinin fluoroprobe reveals multiple sites of cytokinin perception at plasma membrane and endoplasmic reticulum," Nature Communications, Nature, vol. 11(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-17949-0
    DOI: 10.1038/s41467-020-17949-0
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