IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v11y2020i1d10.1038_s41467-020-17796-z.html
   My bibliography  Save this article

Morphogenesis and cytopathic effect of SARS-CoV-2 infection in human airway epithelial cells

Author

Listed:
  • Na Zhu

    (Chinese Center for Disease Control and Prevention)

  • Wenling Wang

    (Chinese Center for Disease Control and Prevention)

  • Zhidong Liu

    (Capital Medical University (Beijing Tuberculosis and Thoracic Tumor Research Institute))

  • Chaoyang Liang

    (China–Japan Friendship Hospital)

  • Wen Wang

    (Chinese Center for Disease Control and Prevention)

  • Fei Ye

    (Chinese Center for Disease Control and Prevention)

  • Baoying Huang

    (Chinese Center for Disease Control and Prevention)

  • Li Zhao

    (Chinese Center for Disease Control and Prevention)

  • Huijuan Wang

    (Chinese Center for Disease Control and Prevention)

  • Weimin Zhou

    (Chinese Center for Disease Control and Prevention)

  • Yao Deng

    (Chinese Center for Disease Control and Prevention)

  • Longfei Mao

    (Suzhou Institute of Systems Medicine)

  • Chongyu Su

    (Capital Medical University (Beijing Tuberculosis and Thoracic Tumor Research Institute))

  • Guangliang Qiang

    (China–Japan Friendship Hospital)

  • Taijiao Jiang

    (Suzhou Institute of Systems Medicine)

  • Jincun Zhao

    (The First Affiliated Hospital of Guangzhou Medical University
    Guangzhou Eighth People’s Hospital of Guangzhou Medical University)

  • Guizhen Wu

    (Chinese Center for Disease Control and Prevention)

  • Jingdong Song

    (Chinese Center for Disease Control and Prevention
    Chinese Center for Disease Control and Prevention)

  • Wenjie Tan

    (Chinese Center for Disease Control and Prevention
    Chinese Academy of Sciences)

Abstract

SARS-CoV-2, a β-coronavirus, has rapidly spread across the world, highlighting its high transmissibility, but the underlying morphogenesis and pathogenesis remain poorly understood. Here, we characterize the replication dynamics, cell tropism and morphogenesis of SARS-CoV-2 in organotypic human airway epithelial (HAE) cultures. SARS-CoV-2 replicates efficiently and infects both ciliated and secretory cells in HAE cultures. In comparison, HCoV-NL63 replicates to lower titers and is only detected in ciliated cells. SARS-CoV-2 shows a similar morphogenetic process as other coronaviruses but causes plaque-like cytopathic effects in HAE cultures. Cell fusion, apoptosis, destruction of epithelium integrity, cilium shrinking and beaded changes are observed in the plaque regions. Taken together, our results provide important insights into SARS-CoV-2 cell tropism, replication and morphogenesis.

Suggested Citation

  • Na Zhu & Wenling Wang & Zhidong Liu & Chaoyang Liang & Wen Wang & Fei Ye & Baoying Huang & Li Zhao & Huijuan Wang & Weimin Zhou & Yao Deng & Longfei Mao & Chongyu Su & Guangliang Qiang & Taijiao Jiang, 2020. "Morphogenesis and cytopathic effect of SARS-CoV-2 infection in human airway epithelial cells," Nature Communications, Nature, vol. 11(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-17796-z
    DOI: 10.1038/s41467-020-17796-z
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-020-17796-z
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-020-17796-z?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Mark E. Becker & Laura Martin-Sancho & Lacy M. Simons & Michael D. McRaven & Sumit K. Chanda & Judd F. Hultquist & Thomas J. Hope, 2024. "Live imaging of airway epithelium reveals that mucociliary clearance modulates SARS-CoV-2 spread," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    2. Junying Wang & Wenting Lu & Jerry Zhang & Yong Du & Mingli Fang & Ao Zhang & Gabriel Sungcad & Samantha Chon & Junji Xing, 2024. "Loss of TRIM29 mitigates viral myocarditis by attenuating PERK-driven ER stress response in male mice," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    3. Catherine F. Hatton & Rachel A. Botting & Maria Emilia Dueñas & Iram J. Haq & Bernard Verdon & Benjamin J. Thompson & Jarmila Stremenova Spegarova & Florian Gothe & Emily Stephenson & Aaron I. Gardner, 2021. "Delayed induction of type I and III interferons mediates nasal epithelial cell permissiveness to SARS-CoV-2," Nature Communications, Nature, vol. 12(1), pages 1-17, December.
    4. Andreia L. Pinto & Ranjit K. Rai & Jonathan C. Brown & Paul Griffin & James R. Edgar & Anand Shah & Aran Singanayagam & Claire Hogg & Wendy S. Barclay & Clare E. Futter & Thomas Burgoyne, 2022. "Ultrastructural insight into SARS-CoV-2 entry and budding in human airway epithelium," Nature Communications, Nature, vol. 13(1), pages 1-14, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-17796-z. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.