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Morphogenesis and cytopathic effect of SARS-CoV-2 infection in human airway epithelial cells

Author

Listed:
  • Na Zhu

    (Chinese Center for Disease Control and Prevention)

  • Wenling Wang

    (Chinese Center for Disease Control and Prevention)

  • Zhidong Liu

    (Capital Medical University (Beijing Tuberculosis and Thoracic Tumor Research Institute))

  • Chaoyang Liang

    (China–Japan Friendship Hospital)

  • Wen Wang

    (Chinese Center for Disease Control and Prevention)

  • Fei Ye

    (Chinese Center for Disease Control and Prevention)

  • Baoying Huang

    (Chinese Center for Disease Control and Prevention)

  • Li Zhao

    (Chinese Center for Disease Control and Prevention)

  • Huijuan Wang

    (Chinese Center for Disease Control and Prevention)

  • Weimin Zhou

    (Chinese Center for Disease Control and Prevention)

  • Yao Deng

    (Chinese Center for Disease Control and Prevention)

  • Longfei Mao

    (Suzhou Institute of Systems Medicine)

  • Chongyu Su

    (Capital Medical University (Beijing Tuberculosis and Thoracic Tumor Research Institute))

  • Guangliang Qiang

    (China–Japan Friendship Hospital)

  • Taijiao Jiang

    (Suzhou Institute of Systems Medicine)

  • Jincun Zhao

    (The First Affiliated Hospital of Guangzhou Medical University
    Guangzhou Eighth People’s Hospital of Guangzhou Medical University)

  • Guizhen Wu

    (Chinese Center for Disease Control and Prevention)

  • Jingdong Song

    (Chinese Center for Disease Control and Prevention
    Chinese Center for Disease Control and Prevention)

  • Wenjie Tan

    (Chinese Center for Disease Control and Prevention
    Chinese Academy of Sciences)

Abstract

SARS-CoV-2, a β-coronavirus, has rapidly spread across the world, highlighting its high transmissibility, but the underlying morphogenesis and pathogenesis remain poorly understood. Here, we characterize the replication dynamics, cell tropism and morphogenesis of SARS-CoV-2 in organotypic human airway epithelial (HAE) cultures. SARS-CoV-2 replicates efficiently and infects both ciliated and secretory cells in HAE cultures. In comparison, HCoV-NL63 replicates to lower titers and is only detected in ciliated cells. SARS-CoV-2 shows a similar morphogenetic process as other coronaviruses but causes plaque-like cytopathic effects in HAE cultures. Cell fusion, apoptosis, destruction of epithelium integrity, cilium shrinking and beaded changes are observed in the plaque regions. Taken together, our results provide important insights into SARS-CoV-2 cell tropism, replication and morphogenesis.

Suggested Citation

  • Na Zhu & Wenling Wang & Zhidong Liu & Chaoyang Liang & Wen Wang & Fei Ye & Baoying Huang & Li Zhao & Huijuan Wang & Weimin Zhou & Yao Deng & Longfei Mao & Chongyu Su & Guangliang Qiang & Taijiao Jiang, 2020. "Morphogenesis and cytopathic effect of SARS-CoV-2 infection in human airway epithelial cells," Nature Communications, Nature, vol. 11(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-17796-z
    DOI: 10.1038/s41467-020-17796-z
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    Cited by:

    1. Junying Wang & Wenting Lu & Jerry Zhang & Yong Du & Mingli Fang & Ao Zhang & Gabriel Sungcad & Samantha Chon & Junji Xing, 2024. "Loss of TRIM29 mitigates viral myocarditis by attenuating PERK-driven ER stress response in male mice," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    2. Catherine F. Hatton & Rachel A. Botting & Maria Emilia Dueñas & Iram J. Haq & Bernard Verdon & Benjamin J. Thompson & Jarmila Stremenova Spegarova & Florian Gothe & Emily Stephenson & Aaron I. Gardner, 2021. "Delayed induction of type I and III interferons mediates nasal epithelial cell permissiveness to SARS-CoV-2," Nature Communications, Nature, vol. 12(1), pages 1-17, December.
    3. Andreia L. Pinto & Ranjit K. Rai & Jonathan C. Brown & Paul Griffin & James R. Edgar & Anand Shah & Aran Singanayagam & Claire Hogg & Wendy S. Barclay & Clare E. Futter & Thomas Burgoyne, 2022. "Ultrastructural insight into SARS-CoV-2 entry and budding in human airway epithelium," Nature Communications, Nature, vol. 13(1), pages 1-14, December.

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