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Quantitative evaluation of protective antibody response induced by hepatitis E vaccine in humans

Author

Listed:
  • Gui-Ping Wen

    (Xiamen University)

  • Linling He

    (The Scripps Research Institute)

  • Zi-Min Tang

    (Xiamen University)

  • Si-Ling Wang

    (Xiamen University)

  • Xu Zhang

    (Xiamen University)

  • Yuan-Zhi Chen

    (Xiamen University)

  • Xiaohe Lin

    (The Scripps Research Institute)

  • Chang Liu

    (Xiamen University
    Xiamen University)

  • Jia-Xin Chen

    (Xiamen University)

  • Dong Ying

    (Xiamen University
    Xiamen University)

  • Zi-Hao Chen

    (Xiamen University)

  • Ying-Bin Wang

    (Xiamen University)

  • Wen-Xin Luo

    (Xiamen University
    Xiamen University)

  • Shou-Jie Huang

    (Xiamen University)

  • Shao-Wei Li

    (Xiamen University
    Xiamen University)

  • Jun Zhang

    (Xiamen University)

  • Zi-Zheng Zheng

    (Xiamen University)

  • Jiang Zhu

    (The Scripps Research Institute
    The Scripps Research Institute)

  • Ning-Shao Xia

    (Xiamen University
    Xiamen University)

Abstract

Efficacy evaluation through human trials is crucial for advancing a vaccine candidate to clinics. Next-generation sequencing (NGS) can be used to quantify B cell repertoire response and trace antibody lineages during vaccination. Here, we demonstrate this application with a case study of Hecolin®, the licensed vaccine for hepatitis E virus (HEV). Four subjects are administered the vaccine following a standard three-dose schedule. Vaccine-induced antibodies exhibit a high degree of clonal diversity, recognize five conformational antigenic sites of the genotype 1 HEV p239 antigen, and cross-react with other genotypes. Unbiased repertoire sequencing is performed for seven time points over six months of vaccination, with maturation pathways characterize for a set of vaccine-induced antibodies. In addition to dynamic repertoire profiles, NGS analysis reveals differential patterns of HEV-specific antibody lineages and highlights the necessity of the long vaccine boost. Together, our study presents a quantitative strategy for vaccine evaluation in small-scale human studies.

Suggested Citation

  • Gui-Ping Wen & Linling He & Zi-Min Tang & Si-Ling Wang & Xu Zhang & Yuan-Zhi Chen & Xiaohe Lin & Chang Liu & Jia-Xin Chen & Dong Ying & Zi-Hao Chen & Ying-Bin Wang & Wen-Xin Luo & Shou-Jie Huang & Sha, 2020. "Quantitative evaluation of protective antibody response induced by hepatitis E vaccine in humans," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-17737-w
    DOI: 10.1038/s41467-020-17737-w
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    Cited by:

    1. Yi-Zong Lee & Jerome Han & Yi-Nan Zhang & Garrett Ward & Keegan Braz Gomes & Sarah Auclair & Robyn L. Stanfield & Linling He & Ian A. Wilson & Jiang Zhu, 2024. "Rational design of uncleaved prefusion-closed trimer vaccines for human respiratory syncytial virus and metapneumovirus," Nature Communications, Nature, vol. 15(1), pages 1-25, December.

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